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Structural and functional effects of oleic acid and iontophoresis on hairless mouse stratum corneum

 Shao Jun Jiang  ;  Sang Min Hwang  ;  Eung Ho Choi  ;  Peter M. Elias  ;  Sung Ku Ahn  ;  Seung Hun Lee 
 Journal of Investigative Dermatology, Vol.114(1) : 64-70, 2000 
Journal Title
 Journal of Investigative Dermatology 
Issue Date
Animals ; Epidermis/metabolism ; Iontophoresis ; Kinetics ; Male ; Mice ; Mice, Hairless ; Microscopy, Electron ; Oleic Acid/pharmacology* ; Permeability/drug effects ; Skin/drug effects* ; Skin/metabolism ; Skin/ultrastructure* ; Water Loss, Insensible/drug effects
barrier function ; intercellular lipid lamellae ; transdermal drug delivery
The aim of this study was to assess the effects of chemical and electrical modes of percutaneous penetration enhancement on the intercellular lipid lamellae of the stratum corneum. Hairless mice were treated with either oleic acid/propylene glycol and iontophoresis separately or together. Permeability barrier function was evaluated by measuring transepidermal water loss and correlated with the structure of stratum corneum intercellular lamellae, as evaluated by electron microscopy, using ruthenium tetroxide postfixation. Transepidermal water loss levels did not change following 1 h iontophoresis alone. In contrast, topical applications of 0.3 M oleic acid in propylene glycol for 1 h increased transepidermal water loss significantly. Moreover, the combined use of iontophoresis plus 0.3 M oleic acid for 1 h further increased transepidermal water loss at equivalent time points. Ultrastructural observations demonstrated both marked disorganization of the intercellular lipid lamellae, as well as the presence of distended lacunae within the stratum corneum in oleic acid/propylene glycol plus or minus iontophoresis-treated stratum corneum. This study provides direct evidence that the oleic acid/propylene glycol system can disrupt the stratum corneum lipid lamellar structures, and that coapplications of oleic acid with iontophoresis further enhance the effects of oleic acid. The synergy between chemical and physical enhancement may afford a new approach to promote transdermal drug delivery.
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1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Seung Hun(이승헌)
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