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Suppression of NF-kappaB activation and cytokine production by N-acetylcysteine in pancreatic acinar cells.
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.date.accessioned | 2019-11-11T05:08:03Z | - |
dc.date.available | 2019-11-11T05:08:03Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 0891-5849 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/171638 | - |
dc.description.abstract | Reactive oxygen species (ROS), generated by infiltrating neutrophils, are considered as an important regulator in the pathogenesis and development of pancreatitis. A hallmark of the inflammatory response is the induction of cytokine gene expression, which may be regulated by oxidant-sensitive transcription factor, nuclear factor-kappaB (NF-kappaB). Present study aims to investigate whether neutrophils primed by 4beta-phorbol 12beta-myristate 13alpha-acetate (PMA) affect the productions of H(2)O(2) and lipid peroxide (LPO), NF-kappaB activation and cytokine production in pancreatic acinar cells, and whether these alterations were inhibited by N-acetylcysteine (NAC) and superoxide dismutase (SOD). Neutrophils generated ROS by stimulation with PMA, which was inhibited by NAC and SOD. In acinar cells, PMA-primed neutrophils increased the productions of H(2)O(2), LPO, and cytokines both time and dose dependently. PMA-primed neutrophils resulted in the activation of two species of NF-kappaB dimers (a p50/p65 heterodimer and a p50 homodimer) in acinar cells. Both NAC and SOD inhibited neutrophil-induced, oxidant-mediated alterations in acinar cells. In conclusion, ROS, generated by neutrophils, activates NF-kappaB, resulting in upregulation of inflammatory cytokines in acinar cells. Antioxidants such as NAC might be useful antiinflammatory agents by inhibiting oxidant-mediated activation of NF-kappaB and decreasing cytokine production. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier Science | - |
dc.relation.isPartOf | Free Radical Biology and Medicine | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Acetylcysteine/pharmacology* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antioxidants/pharmacology* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Cytokines/analysis | - |
dc.subject.MESH | Cytokines/genetics* | - |
dc.subject.MESH | Gene Expression Regulation/drug effects | - |
dc.subject.MESH | Gene Expression Regulation/immunology* | - |
dc.subject.MESH | Hydrogen Peroxide/metabolism | - |
dc.subject.MESH | Kinetics | - |
dc.subject.MESH | Lipid Peroxidation/drug effects* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | NF-kappa B/antagonists & inhibitors* | - |
dc.subject.MESH | Pancreas/drug effects | - |
dc.subject.MESH | Pancreas/immunology | - |
dc.subject.MESH | Pancreas/physiology* | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Superoxide Dismutase/metabolism | - |
dc.subject.MESH | Tetradecanoylphorbol Acetate/pharmacology | - |
dc.subject.MESH | Time Factors | - |
dc.subject.MESH | Transcription, Genetic/drug effects | - |
dc.title | Suppression of NF-kappaB activation and cytokine production by N-acetylcysteine in pancreatic acinar cells. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학교실) | - |
dc.contributor.googleauthor | Hyeyoung Kim | - |
dc.contributor.googleauthor | Jeong Yeon Seo | - |
dc.contributor.googleauthor | Kwan Ho Roh | - |
dc.contributor.googleauthor | Joo Weon Lim | - |
dc.contributor.googleauthor | Kyung Hwan Kim | - |
dc.identifier.doi | 10.1016/S0891-5849(00)00368-3 | - |
dc.contributor.localId | A00311 | - |
dc.relation.journalcode | J00906 | - |
dc.identifier.eissn | 1873-4596 | - |
dc.identifier.pmid | 11033420 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0891584900003683 | - |
dc.subject.keyword | Reactive oxygen species | - |
dc.subject.keyword | NF-κB | - |
dc.subject.keyword | Cytokine | - |
dc.subject.keyword | Pancreatic acinar cells | - |
dc.subject.keyword | Neutrophils | - |
dc.subject.keyword | N-acetylcysteine | - |
dc.subject.keyword | Free radicals | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.affiliatedAuthor | 김경환 | - |
dc.citation.volume | 29 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 674 | - |
dc.citation.endPage | 683 | - |
dc.identifier.bibliographicCitation | Free Radical Biology and Medicine, Vol.29(7) : 674-683, 2000 | - |
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