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Identification of new Single-Nucleotide Polymorphisns in the Thrombin Receptor Gene and their Effect on Coronary Artery Diseases in Korean.

 H‐Y Park  ;  T Nabika  ;  Y Jang  ;  D Kim  ;  H‐S Kim  ;  J Masuda 
 Clinical and Experimental Pharmacology and Physiology, Vol.27(9) : 690-693, 2000 
Journal Title
 Clinical and Experimental Pharmacology and Physiology 
Issue Date
5' Untranslated Regions/genetics ; Adult ; Coronary Disease/genetics* ; Female ; Genetic Predisposition to Disease ; Genetic Variation ; Genotype Haplotypes ; Humans ; Male ; Middle Aged ; Polymorphism, Single Nucleotide* ; Receptor, PAR-1 ; Receptors, Thrombin/genetics* ; Regulatory ; Sequences, Nucleic Acid ; Repetitive Sequences, Nucleic Acid
association study ; coronary artery disease ; genetics ; thrombin receptor
1. The thrombin receptor (the protease-activated receptor-1; PAR-1) is located on vascular cells as well as platelets and may play important roles in atherosclerotic disorders, such as coronary artery diseases (CAD). In the present study, we searched for genetic polymorphisms of the PAR-1 gene and evaluated their effects on CAD by association analysis. 2. We identified six polymorphisms in the 5'-untranslated region of the PAR-1 gene by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP); five single-nucleotide polymorphisms (SNP) at -2355 (A to G), -2333 (T to G), -1428 (G to A), -1071 (C to T) and -561 (A to G) and a simple sequence repeat (SSR) polymorphism between -1935 and -1841. Five SNP were in strong linkage disequilibrium with each other to make three major haplotypes, the frequency of which was over 90% of all possible haplotypes. 3. For association analysis, 150 patients who had CAD (CAD+), 58 subjects who had no stenosis on the coronary angiogram and 186 reference subjects who had no clinical evidence of CAD were used from the Korean population. The genotype frequencies of the SNP were in Hardy-Weinberg equilibrium, except A-561G in CAD+. The association of these SNP as well as of the SSR with CAD was not evident. This result suggests no major roles of the PAR-1 gene in CAD in Koreans.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Jang, Yang Soo(장양수) ORCID logo https://orcid.org/0000-0002-2169-3112
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