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Modification of cycloserine cefoxitin fructose agar to supress growth of yeasts from stool specimens

DC Field Value Language
dc.contributor.author염종화-
dc.contributor.author용동은-
dc.contributor.author이경원-
dc.contributor.author용동은-
dc.contributor.author이경원-
dc.date.accessioned2019-11-11T05:00:57Z-
dc.date.available2019-11-11T05:00:57Z-
dc.date.issued2000-
dc.identifier.issn1075-9964-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/171542-
dc.description.abstractCycloserine cefoxitin fructose agar is a widely used selective isolation medium for Clostridium difficile from stool specimens. Yeasts often colonize in the intestine of C. difficile disease patients and, if colonized heavily, pure culture of C. difficile can be delayed. The aim of this study was to modify cycloserine cefoxitin fructose agar to suppress the growth of yeasts. Antimicrobial activities of three commonly available antifungal agents were tested against recent clinical isolates of Candida species. Amphotericin B was most active in inhibiting all isolates by 0.5mg/L concentration. Cycloserine cefoxitin fructose agar was modified by adding 2mg/L of amphotericin B. Serial ten-fold dilution of stool specimens from 126 suspected C. difficile-associated diarrhea patients were cultured both on cycloserine cefoxitin fructose agar plates and modified agar plates. Yeasts grew from 60 specimens on cycloserine cefoxitin fructose agar, but none grew on the modified medium. Growth of C. difficile was detected from 37 and 39 of 126 specimens on cycloserine cefoxitin fructose agar and modified medium, respectively. The number of C. difficile colonies was similar on both media. In conclusion, 2mg/L of amphotericin B supplementation to cycloserine cefoxitin fructose agar can facilitate the isolation of C. difficile from stool specimens which are densely colonized with yeasts.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherAcademic Press-
dc.relation.isPartOfAnaerobe-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleModification of cycloserine cefoxitin fructose agar to supress growth of yeasts from stool specimens-
dc.typeArticle-
dc.contributor.collegeResearch Institutes (연구소)-
dc.contributor.departmentResearch Institute of Bacterial Resistance (세균내성연구소)-
dc.contributor.googleauthorKyungwon Lee-
dc.contributor.googleauthorDongeunYong-
dc.contributor.googleauthorJonghwaYum-
dc.contributor.googleauthorYoung Suk Sohn-
dc.contributor.googleauthorYunsop Chong-
dc.identifier.doi10.1006/anae.2000.0355-
dc.contributor.localIdA02352-
dc.contributor.localIdA02423-
dc.contributor.localIdA02423-
dc.contributor.localIdA02649-
dc.contributor.localIdA02649-
dc.contributor.localIdA02423-
dc.contributor.localIdA02423-
dc.contributor.localIdA02649-
dc.contributor.localIdA02649-
dc.relation.journalcodeJ02967-
dc.identifier.eissn1095-8274-
dc.subject.keywordC. difficile-
dc.subject.keywordCCFA-
dc.subject.keywordamphotericin B-supplemented CCFA-
dc.subject.keywordyeast suppression-
dc.contributor.alternativeNameYum, Jong Hwa-
dc.contributor.affiliatedAuthor염종화-
dc.contributor.affiliatedAuthor용동은-
dc.contributor.affiliatedAuthor용동은-
dc.contributor.affiliatedAuthor이경원-
dc.contributor.affiliatedAuthor이경원-
dc.contributor.affiliatedAuthor용동은-
dc.contributor.affiliatedAuthor용동은-
dc.contributor.affiliatedAuthor이경원-
dc.contributor.affiliatedAuthor이경원-
dc.citation.volume6-
dc.citation.number5-
dc.citation.startPage269-
dc.citation.endPage271-
dc.identifier.bibliographicCitationAnaerobe, Vol.6(5) : 269-271, 2000-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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