Cited 32 times in
MicroRNA-27 inhibits adipogenic differentiation in orbital fibroblasts from patients with Graves' orbitopathy
DC Field | Value | Language |
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dc.contributor.author | 윤진숙 | - |
dc.contributor.author | 이은직 | - |
dc.date.accessioned | 2019-10-28T01:41:20Z | - |
dc.date.available | 2019-10-28T01:41:20Z | - |
dc.date.issued | 2019 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/171297 | - |
dc.description.abstract | BACKGROUND: To investigate the role of microRNA (miR)-27a and miR-27b in adipogenesis in an in vitro model of Graves' orbitopathy (GO). METHODS: Orbital fat tissues were harvested from GO and non-GO participants for primary orbital fibroblast cultures. The expression levels of miR-27a and miR-27b between GO and non-GO orbital fat tissues were compared. During adipogenesis of GO orbital fibroblasts, the expression levels of miR-27a and miR-27b were determined, and the effects of mimics of miR-27a and miR-27b transfection on adipogenesis of GO orbital fibroblast were investigated. RESULTS: Real time-polymerase chain reaction showed significantly more decreases in miR-27a and miR-27b levels in orbital fat tissues in GO participants than in non-GO participants (p < 0.05). The expression of both miR-27a and miR-27b was highest in orbital fibroblasts at day 0 and declined gradually after the induction of adipogenic differentiation. The expression levels of PPARγ, CCAAT/enhancer binding protein (C/EBP)α and C/EBPβ were decreased and Oil Red O-stained lipid droplets were lower in GO orbital fibroblasts transfected with miR-27a and miR-27b mimics than in negative controls. CONCLUSIONS: Our results indicated that miR-27a and miR-27b inhibited adipogenesis in orbital fibroblasts from GO patients. Further studies are required to examine the potential of miR-27a and miR-27b as targets for therapeutic strategies. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Public Library of Science | - |
dc.relation.isPartOf | PLoS One | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | MicroRNA-27 inhibits adipogenic differentiation in orbital fibroblasts from patients with Graves' orbitopathy | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학교실) | - |
dc.contributor.googleauthor | Sun Young Jang | - |
dc.contributor.googleauthor | Min Kyung Chae | - |
dc.contributor.googleauthor | Joon H. Lee | - |
dc.contributor.googleauthor | Eun Jig Lee | - |
dc.contributor.googleauthor | Jin Sook Yoon | - |
dc.identifier.doi | 10.1371/journal.pone.0221077 | - |
dc.contributor.localId | A02611 | - |
dc.contributor.localId | A03050 | - |
dc.relation.journalcode | J02540 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.pmid | 31415657 | - |
dc.contributor.alternativeName | Yoon, Jin Sook | - |
dc.contributor.affiliatedAuthor | 윤진숙 | - |
dc.contributor.affiliatedAuthor | 이은직 | - |
dc.citation.volume | 14 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | e0221077 | - |
dc.identifier.bibliographicCitation | PLoS One, Vol.14(8) : e0221077, 2019 | - |
dc.identifier.rimsid | 63897 | - |
dc.type.rims | ART | - |
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