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Clinical and microbiologic investigation of an expedited peri-implantitis dog model: an animal study

Authors
 Wook Jin Seong  ;  Georgios Kotsakis  ;  Jong-Ki Huh  ;  Soo Cheol Jeong  ;  Ki Young Nam  ;  Jong Ryul Kim  ;  Young Cheul Heo  ;  Hyeon-Cheol Kim  ;  Lei Zhang  ;  Michael D. Evans  ;  Heather Conrad  ;  Robert J. Schumacher 
Citation
 BMC Oral Health, Vol.19(1) : 150, 2019 
Journal Title
 BMC Oral Health 
Issue Date
2019
Keywords
Dental implant ; Expedited dog model ; Peri-implantitis
Abstract
BACKGROUND: Animal studies are pivotal in allowing experimentation to identify efficacious treatment protocols for resolution of peri-implantitis. The purpose of this investigation was to characterize an expedited dog peri-implantitis model clinically, radiographically, and microbiologically. METHODS: Eight hound dogs underwent extractions (week 0) and implant (3.3 × 8.5 mm) placement with simultaneous surgical defect creation and ligature placement for induction of peri-implantitis (week 10). Ligatures were replaced at 6 weeks (week 16) and removed after 9 weeks (week 19) when supporting bone loss involved approximately 50% of the peri-implant bone. Microbial samples from the defects and healthy control implant sites collected at week 19 were analyzed utilizing a microarray. Clinical measures of inflammation were obtained and radiographic bone loss was measured from periapical radiographs. Radiographic depth and width measurements of bony defect were repeated at weeks 10 (baseline), 16, and 19. Canonical analysis of principal coordinates was used to visualize overall differences in microbial abundance between peri-implantitis and healthy implants. RESULTS: This accelerated disease protocol led to intrabony defect creation with a mean depth and width of 4.3 mm and 3.5 mm, respectively after 9 weeks of ligature placement. Microbial identification revealed 59 total bacteria in peri-implant sites, 21 of which were only present in peri-implant sites as compared to healthy controls. Overall microbial beta diversity (microbial between-sample compositional diversity) differed between peri-implantitis and healthy implants (p = 0.009). CONCLUSIONS: Within the limitations of this study, this protocol led to expedited generation of peri-implant defects with a microbial profile indicative of a shift to disease and defect patterns conducive to regenerative treatment. However, the possibility of potential spontaneous resolution of lesions due to the lack of a chronicity interval as compared to chronic disease models need to be further clarified and considered during preclinical peri-implantitis model selection.
Files in This Item:
T201902958.pdf Download
DOI
10.1186/s12903-019-0837-y
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
Yonsei Authors
Huh, Jong Ki(허종기) ORCID logo https://orcid.org/0000-0002-7381-3972
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171134
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