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Highly Multiplexed Fluorescence in Situ Hybridization for in Situ Genomics

Authors
 Maristela L. Onozato  ;  Clarence Yapp  ;  Douglas Richardson  ;  Tilak Sundaresan  ;  Varun Chahal  ;  Jesse Lee  ;  James P. Sullivan  ;  Marisa W. Madden  ;  Hyo S. Shim  ;  Matthew Liebers  ;  Quan Ho  ;  Shyamala Maheswaran  ;  Daniel A. Haber  ;  Zongli Zheng  ;  Brian Clancy  ;  Hunter L. Elliott  ;  Jochen K. Lennerz  ;  A. John Iafrate 
Citation
 Journal of Molecular Diagnostics, Vol.21(3) : 390-407, 2019 
Journal Title
 Journal of Molecular Diagnostics 
ISSN
 1525-1578 
Issue Date
2019
Abstract
The quantification of changes in gene copy number is critical to our understanding of tumor biology and for the clinical management of cancer patients. DNA fluorescence in situ hybridization is the gold standard method to detect copy number alterations, but it is limited by the number of genes one can quantify simultaneously. To increase the throughput of this informative technique, a fluorescent bar-code system for the unique labeling of dozens of genes and an automated image analysis algorithm that enabled their simultaneous hybridization for the quantification of gene copy numbers were devised. We demonstrate the reliability of this multiplex approach on normal human lymphocytes, metaphase spreads of transformed cell lines, and cultured circulating tumor cells. It also opens the door to the development of gene panels for more comprehensive analysis of copy number changes in tissue, including the study of heterogeneity and of high-throughput clinical assays that could provide rapid quantification of gene copy numbers in samples with limited cellularity, such as circulating tumor cells.
Full Text
https://www.sciencedirect.com/science/article/pii/S1525157818300229
DOI
10.1016/j.jmoldx.2019.01.010
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Shim, Hyo Sup(심효섭) ORCID logo https://orcid.org/0000-0002-5718-3624
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/170981
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