Cited 90 times in
Particulate matter induces inflammatory cytokine production via activation of NFκB by TLR5-NOX4-ROS signaling in human skin keratinocyte and mouse skin
DC Field | Value | Language |
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dc.contributor.author | 박창욱 | - |
dc.contributor.author | 현영민 | - |
dc.date.accessioned | 2019-09-20T07:26:43Z | - |
dc.date.available | 2019-09-20T07:26:43Z | - |
dc.date.issued | 2019 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/170900 | - |
dc.description.abstract | Particulate matter (PM) increases levels of pro-inflammatory cytokines, but its effects on the skin remain largely unknown. We investigated the signal transduction pathway and epigenetic regulatory mechanisms underlying cellular inflammation induced by PM with a diameter of ≤ 2.5 (PM2.5) in vitro and in vivo. PM2.5-treated skin keratinocytes produced various inflammatory cytokines, including IL-6. The binding of PM2.5 to TLR5 initiated intracellular signaling through MyD88, and led to the translocation of NFκB to the nucleus, where it bound the NFκB site within IL-6 promoter. Furthermore, PM2.5 induced a direct interaction between TLR5 and NOX4, and in turn induced the production of ROS and activated NFκB-IL-6 downstream, which was prevented by siRNA-mediated knockdown of NOX4 or antioxidant treatment. Furthermore, expression of TLR5, MyD88, NOX4, phospho-NFκB, and IL-6 was increased in skin tissue of PM2.5-treated flaky tail mice. PM2.5-induced increased transcription of IL-6 was regulated via DNA methylation and histone methylation by epigenetic modification; the binding of DNA demethylase and histone methyltransferase to the IL-6 promoter regions resulted in increased IL-6 mRNA expression. Our findings provide deep insight into the pathogenesis of PM2.5 exposure and can be used as a therapeutic strategy to treat inflammatory skin diseases caused by PM2.5 exposure. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.relation.isPartOf | Redox Biology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Particulate matter induces inflammatory cytokine production via activation of NFκB by TLR5-NOX4-ROS signaling in human skin keratinocyte and mouse skin | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Dermatology (피부과학교실) | - |
dc.contributor.googleauthor | Yea Seong Ryu | - |
dc.contributor.googleauthor | Kyoung Ah Kang | - |
dc.contributor.googleauthor | Mei Jing Piao | - |
dc.contributor.googleauthor | Mee Jung Ahn | - |
dc.contributor.googleauthor | Joo Mi Yi | - |
dc.contributor.googleauthor | Young-Min Hyun | - |
dc.contributor.googleauthor | Seo Hyeong Kim | - |
dc.contributor.googleauthor | Min Kyung Ko | - |
dc.contributor.googleauthor | Chang Ook Park | - |
dc.contributor.googleauthor | Jin Won Hyun | - |
dc.identifier.doi | 10.1016/j.redox.2018.101080 | - |
dc.contributor.localId | A01716 | - |
dc.contributor.localId | A04814 | - |
dc.relation.journalcode | J03622 | - |
dc.identifier.eissn | 2213-2317 | - |
dc.identifier.pmid | 30584981 | - |
dc.subject.keyword | Epigenetic modification | - |
dc.subject.keyword | Interleukin-6 | - |
dc.subject.keyword | Particulate matter | - |
dc.subject.keyword | Reactive oxygen species | - |
dc.subject.keyword | Toll like receptor | - |
dc.contributor.alternativeName | Park, Chang Ook | - |
dc.contributor.affiliatedAuthor | 박창욱 | - |
dc.contributor.affiliatedAuthor | 현영민 | - |
dc.citation.volume | 21 | - |
dc.citation.startPage | 101080 | - |
dc.identifier.bibliographicCitation | Redox Biology, Vol.21 : 101080, 2019 | - |
dc.identifier.rimsid | 64303 | - |
dc.type.rims | ART | - |
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