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Molecular subtypes of colorectal cancer in pre-clinical models show differential response to targeted therapies: Treatment implications beyond KRAS mutations

Authors
 Rekha Pal  ;  Ning Wei  ;  Nan Song  ;  Shaoyu Wu  ;  Rim S. Kim  ;  Ying Wang  ;  Patrick G. Gavin  ;  Peter C. Lucas  ;  Ashok Srinivasan  ;  Carmen J. Allegra  ;  Samuel A. Jacobs  ;  Soonmyung Paik  ;  John C. Schmitz  ;  Katherine L. Pogue-Geile 
Citation
 PLoS One, Vol.13(8) : e0200836, 2018 
Journal Title
 PLoS One 
Issue Date
2018
MeSH
Animals ; Antineoplastic Combined Chemotherapy Protocols ; Cell Line, Tumor ; Colorectal Neoplasms/drug therapy* ; Colorectal Neoplasms/genetics* ; Colorectal Neoplasms/metabolism ; ErbB Receptors/antagonists & inhibitors ; Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors ; Female ; Humans ; Mice ; Mice, Nude ; Mitogen-Activated Protein Kinase Kinases/antagonists & inhibitors ; Molecular Targeted Therapy ; Mutation* ; Neoplastic Stem Cells/drug effects ; Neoplastic Stem Cells/pathology ; Protein Kinase Inhibitors/therapeutic use ; Proto-Oncogene Proteins p21(ras)/genetics* ; Xenograft Model Antitumor Assays
Abstract
Molecular subtypes of colorectal tumors are associated with prognosis and prediction for treatment benefit from chemotherapy. The purpose of this study was two-fold: 1) to determine the association of colorectal (CRC) molecular subtypes with response to targeted therapies in pre-clinical models and 2) to identify treatments for CRC stem-like subtype because these tumors are associated with a very poor patient prognosis. Eleven CRC cell lines were classified into molecular subtypes and tested for their response to pan-ERBB, MEK, and ERK inhibitors as single agents and in combination. All six inflammatory or TA cell lines were exquisitely sensitive to the combination of MEK and neratinib whereas all five stem-like cell lines were resistant. Growth inhibition in sensitive cell lines was greater with the combination than with either drug alone even in cell lines with KRAS mutations. The combination inhibited pERK in inflammatory cell lines but not in four out of five stem-like cell lines. MEK162 plus neratinib were synergistic in cell culture and xenograft models in inflammatory cell lines. The ERK inhibitor, SCH772984, down-regulated pERK, decreased cell viability, and was synergistic with neratinib in both inflammatory and stem-like subtypes. These results suggest that inhibition of pERK is a critical node in decreasing cell viability of stem-like CRC tumors. Our results also suggest that CRC molecular subtypes may yield predictive information and may help to identify patients who may respond to targeted inhibitors.
DOI
10.1371/journal.pone.0200836
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Paik, Soon Myung(백순명) ORCID logo https://orcid.org/0000-0001-9688-6480
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/170895
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