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En bloc and segmental deletions of human XIST reveal X chromosome inactivation-involving RNA elements

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dc.contributor.author김형범-
dc.contributor.author라무고파라파-
dc.date.accessioned2019-07-23T06:40:09Z-
dc.date.available2019-07-23T06:40:09Z-
dc.date.issued2019-
dc.identifier.issn0305-1048-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/170262-
dc.description.abstractTheXISTRNAis a non-codingRNAthat inducesXchromosomeinactivation (XCI). Unlike the mouseXistRNA, how thehumanXISTRNAcontrols XCI in female cells is less well characterized, and its functional motifs remain unclear. To systematically decipher the XCI-involvingelementsofXISTRNA, 11 smallerXISTsegments, including repeats A, D and E;human-specific repeatelements; the promoter; and non-repetitive exons, as well as the entireXISTgene, were homozygously deleted in K562 cells using the Cas9 nuclease and paired guide RNAs at high efficiencies, followed by high-throughputRNAsequencing andRNAfluorescence in situ hybridization experiments. Clones containingenblocand promoterdeletionsthat consistently displayed noXISTRNAs and a global up-regulation ofX-linked genes confirmed that the deletion ofXISTreactivates the inactiveXchromosome. Systematic analyses ofsegmentaldeletionsdelineated that exon 5 harboring the non-repeat element is important forX-inactivation maintenance, whereas exons 2, 3 and 4 as well as the other repeats in exon 1 are less important, a different situation from that of mouseXist. This Cas9-assisted dissection ofXISTallowed us to understand the unique functional domains within thehumanXISTRNA.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherOxford University Press-
dc.relation.isPartOfNUCLEIC ACIDS RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleEn bloc and segmental deletions of human XIST reveal X chromosome inactivation-involving RNA elements-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학교실)-
dc.contributor.googleauthorHyeon J Lee-
dc.contributor.googleauthorRamu Gopalappa-
dc.contributor.googleauthorHongjae Sunwoo-
dc.contributor.googleauthorSeo-Won Choi Suresh Ramakrishna-
dc.contributor.googleauthorJeannie T Lee-
dc.contributor.googleauthorHyongbum H Kim-
dc.contributor.googleauthorJin-Wu Nam-
dc.identifier.doi10.1093/nar/gkz109-
dc.contributor.localIdA01148-
dc.relation.journalcodeJ02387-
dc.identifier.eissn1362-4962-
dc.identifier.pmid30783652-
dc.contributor.alternativeNameKim, Hyongbum-
dc.contributor.affiliatedAuthor김형범-
dc.citation.volume47-
dc.citation.number8-
dc.citation.startPage3875-
dc.citation.endPage3887-
dc.identifier.bibliographicCitationNUCLEIC ACIDS RESEARCH, Vol.47(8) : 3875-3887, 2019-
dc.identifier.rimsid61987-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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