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Targeted Gene Delivery into the Mammalian Inner Ear Using Synthetic Serotypes of Adeno-Associated Virus Vectors

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dc.contributor.author복진웅-
dc.date.accessioned2019-07-11T03:32:43Z-
dc.date.available2019-07-11T03:32:43Z-
dc.date.issued2019-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/170026-
dc.description.abstractTargeting specific cell types in the mammalian inner ear is important for treating genetic hearing loss due to the different cell type-specific functions. Adeno-associated virus (AAV) is an efficient in vivo gene transfer vector, and it has demonstrated promise for treating genetic hearing loss. Although more than 100 AAV serotypes have been identified, few studies have investigated whether AAV can be distributed to specific inner ear cell types. Here we screened three EGFP-AAV reporter constructs (serotypes DJ, DJ8, and PHP.B) in the neonatal mammalian inner ear by injection via the round window membrane to determine the cellular specificity of the AAV vectors. Sensory hair cells, supporting cells, cells in Reissner's membrane, interdental cells, and root cells were successfully transduced. Hair cells in the cochlear sensory epithelial region were the most frequently transduced cell type by all tested AAV serotypes. The recombinant DJ serotype most effectively transduced a range of cell types at a high rate. Our findings provide a basis for improving treatment of hereditary hearing loss using targeted AAV-mediated gene therapy.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherNature Publishing Group-
dc.relation.isPartOfMOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleTargeted Gene Delivery into the Mammalian Inner Ear Using Synthetic Serotypes of Adeno-Associated Virus Vectors-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Anatomy (해부학교실)-
dc.contributor.googleauthorMin-A Kim-
dc.contributor.googleauthorNari Ryu-
dc.contributor.googleauthorHye-Min Kim-
dc.contributor.googleauthorYe-Ri Kim-
dc.contributor.googleauthorByeonghyeon Lee-
dc.contributor.googleauthorTae-Jun Kwon-
dc.contributor.googleauthorJinwoong Bok-
dc.contributor.googleauthorUn-Kyung Kim-
dc.identifier.doi10.1016/j.omtm.2019.01.002-
dc.contributor.localIdA01865-
dc.relation.journalcodeJ03620-
dc.identifier.eissn2329-0501-
dc.identifier.pmid30805407-
dc.subject.keywordadeno-associated virus-
dc.subject.keywordgene therapy-
dc.subject.keywordgenetic hearing loss-
dc.subject.keywordinner ear-
dc.subject.keywordserotype-
dc.contributor.alternativeNameBok, Jin Woong-
dc.contributor.affiliatedAuthor복진웅-
dc.citation.volume13-
dc.citation.startPage197-
dc.citation.endPage204-
dc.identifier.bibliographicCitationMOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT, Vol.13 : 197-204, 2019-
dc.identifier.rimsid61831-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers

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