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Effects of Different Calcium Silicate Cements on the Inflammatory Response and Odontogenic Differentiation of Lipopolysaccharide-Stimulated Human Dental Pulp Stem Cells

DC FieldValueLanguage
dc.contributor.author김선일-
dc.contributor.author김예림-
dc.contributor.author김욱성-
dc.contributor.author김의성-
dc.date.accessioned2019-07-11T03:11:09Z-
dc.date.available2019-07-11T03:11:09Z-
dc.date.issued2019-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/169869-
dc.description.abstractThis study aimed to analyze the effects of different calcium silicate cements (CSCs) on the inflammatory response and odontogenic differentiation of lipopolysaccharide-stimulated human dental pulp stem cells. Human dental pulp stem cells (hDPSCs) were stimulated with lipopolysaccharide (LPS) to induce inflammation. These LPS-induced dental pulp stem cells (LDPSCs) were cultured with ProRoot MTA, Biodentine, Retro MTA, and Dycal. Cell viability was evaluated using the Cell Counting Kit-8 assay. Interleukin (IL)-6, IL-8, and transforming growth factor (TGF)-β1 cytokine levels were assessed using the enzyme-linked immunosorbent assay. The expressions of alkaline phosphatase (ALP), osteocalcin, and runt-related transcription factor 2 (RUNX2) were analyzed through real-time polymerase chain reaction. ProRoot MTA, Biodentine, and Retro MTA did not significantly decrease the cell viability of LDPSCs for up to 48 h (p < 0.05). Retro MTA significantly decreased the expression of IL-6 and IL-8 by LDPSCs. ProRoot MTA and Biodentine significantly reduced TGF-β expression by LDPSCs (p < 0.05). Regarding odontogenic differentiation, all CSCs had no effect on ALP expression but increased the production of RUNX2 at 12 h.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherMDPI-
dc.relation.isPartOfMATERIALS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleEffects of Different Calcium Silicate Cements on the Inflammatory Response and Odontogenic Differentiation of Lipopolysaccharide-Stimulated Human Dental Pulp Stem Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Conservative Dentistry (보존과학교실)-
dc.contributor.googleauthorMinsun Chung-
dc.contributor.googleauthorSukjoon Lee-
dc.contributor.googleauthorDongzi Chen-
dc.contributor.googleauthorUkseong Kim-
dc.contributor.googleauthorYaelim Kim-
dc.contributor.googleauthorSunil Kim-
dc.contributor.googleauthorEuiseong Kim-
dc.identifier.doi10.3390/ma12081259-
dc.contributor.localIdA00556-
dc.contributor.localIdA05723-
dc.contributor.localIdA05723-
dc.contributor.localIdA05724-
dc.contributor.localIdA05724-
dc.contributor.localIdA00833-
dc.contributor.localIdA00833-
dc.relation.journalcodeJ02182-
dc.identifier.eissn1996-1944-
dc.identifier.pmid30999582-
dc.subject.keywordcalcium silicate cement-
dc.subject.keywordhuman dental pulp stem cell-
dc.subject.keywordinflammation-
dc.subject.keywordlipopolysaccharide-
dc.subject.keywordodontogenic differentiation-
dc.contributor.alternativeNameKim, Sun Il-
dc.contributor.affiliatedAuthor김선일-
dc.contributor.affiliatedAuthor김예림-
dc.contributor.affiliatedAuthor김예림-
dc.contributor.affiliatedAuthor김욱성-
dc.contributor.affiliatedAuthor김욱성-
dc.contributor.affiliatedAuthor김의성-
dc.contributor.affiliatedAuthor김의성-
dc.citation.volume12-
dc.citation.number8-
dc.citation.startPageE1259-
dc.identifier.bibliographicCitationMATERIALS, Vol.12(8) : E1259, 2019-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers

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