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Optogenetic stimulation promotes Schwann cell proliferation, differentiation, and myelination in vitro

DC FieldValueLanguage
dc.contributor.author조성래-
dc.date.accessioned2019-05-29T05:25:45Z-
dc.date.available2019-05-29T05:25:45Z-
dc.date.issued2019-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/169591-
dc.description.abstractSchwann cells (SCs) constitute a crucial element of the peripheral nervous system, by structurally supporting the formation of myelin and conveying vital trophic factors to the nervous system. However, the functions of SCs in developmental and regenerative stages remain unclear. Here, we investigated how optogenetic stimulation (OS) of SCs regulates their development. In SC monoculture, OS substantially enhanced SC proliferation and the number of BrdU+-S100ß+-SCs over time. In addition, OS also markedly promoted the expression of both Krox20 and myelin basic protein (MBP) in SC culture medium containing dBcAMP/NRG1, which induced differentiation. We found that the effects of OS are dependent on the intracellular Ca2+ level. OS induces elevated intracellular Ca2+ levels through the T-type voltage-gated calcium channel (VGCC) and mobilization of Ca2+ from both inositol 1,4,5-trisphosphate (IP3)-sensitive stores and caffeine/ryanodine-sensitive stores. Furthermore, we confirmed that OS significantly increased expression levels of both Krox20 and MBP in SC-motor neuron (MN) coculture, which was notably prevented by pharmacological intervention with Ca2+. Taken together, our results demonstrate that OS of SCs increases the intracellular Ca2+ level and can regulate proliferation, differentiation, and myelination, suggesting that OS of SCs may offer a new approach to the treatment of neurodegenerative disorders.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherNature Publishing Group-
dc.relation.isPartOfSCIENTIFIC REPORTS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleOptogenetic stimulation promotes Schwann cell proliferation, differentiation, and myelination in vitro-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Rehabilitation Medicine (재활의학교실)-
dc.contributor.googleauthorKyuhwan Jung-
dc.contributor.googleauthorJi Hye Park-
dc.contributor.googleauthorSung-Yon Kim-
dc.contributor.googleauthorNoo Li Jeon-
dc.contributor.googleauthorSung-Rae Cho-
dc.contributor.googleauthorSujin Hyung-
dc.identifier.doi10.1038/s41598-019-40173-w-
dc.contributor.localIdA03831-
dc.relation.journalcodeJ02646-
dc.identifier.eissn2045-2322-
dc.identifier.pmid30837563-
dc.contributor.alternativeNameCho, Sung Rae-
dc.contributor.affiliatedAuthor조성래-
dc.citation.volume9-
dc.citation.number1-
dc.citation.startPage3487-
dc.identifier.bibliographicCitationSCIENTIFIC REPORTS, Vol.9(1) : 3487, 2019-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers

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