Cited 8 times in
IL-15 Generates IFN-γ-producing Cells Reciprocally Expressing Lymphoid-Myeloid Markers during Dendritic Cell Differentiation
DC Field | Value | Language |
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dc.contributor.author | 신성재 | - |
dc.contributor.author | 윤주헌 | - |
dc.date.accessioned | 2019-04-03T07:48:34Z | - |
dc.date.available | 2019-04-03T07:48:34Z | - |
dc.date.issued | 2019 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/167754 | - |
dc.description.abstract | Recently, interest in IL-15-differentiated cells has increased; however, the phenotypic definition of IL-15-differentiated bone marrow-derived cells (IL-15-DBMCs) is still under debate, particularly the generation of IFN-γ-producing innate cells such as premature NK (pre-mNK) cells, natural killer dendritic cells (NKDCs), interferon-producing killer dendritic cells (IKDCs), and type 1 innate lymphoid cells (ILC1s), all of which are IL-15-dependent. Here, we revisited the immunophenotypic characteristics of IFN-γ-producing IL-15-DBMCs and their functional role in the control of intracellular Mycobacterium tuberculosis (Mtb) infection. When comparing the cytokine levels between bone marrow-derived dendritic cells (BMDCs) and IL-15-DBMCs upon stimulation with various TLR agonists, only the CD11cint population of IL-15-DBMCs produced significant levels of IFN-γ, decreased levels of MHC-II, and increased levels of B220. Neither BMDCs nor IL-15-DBMCs were found to express DX5 or NK1.1, which are representative markers for the NK cell lineage and IKDCs. When the CD11cintB220+ population of IL-15-DBMCs was enriched, the Thy1.2+Sca-1+ population showed a marked increase in IFN-γ production. In addition, while depletion of the B220+ and Thy1.2+ populations of IL-15-DBMCs, but not the CD19+ population, inhibited IFN-γ production, enrichment of these cell populations increased IFN-γ. Ultimately, co-culture of sorted IFN-γ-producing B220+Thy1.2+ IL-15-DBMCs with Mtb-infected macrophages resulted in control of the intracellular growth of Mtb via the IFN-γ-nitric oxide axis in a donor cell number-dependent manner. Taken together, the results indicate that IFN-γ-producing IL-15-DBMCs could be redefined as CD11cintB220+Thy1.2+Sca-1+ cells, which phenotypically resemble both IKDCs and ILC1s, and may have therapeutic potential for controlling infectious intracellular bacteria such as Mtb. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Ivyspring International | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | IL-15 Generates IFN-γ-producing Cells Reciprocally Expressing Lymphoid-Myeloid Markers during Dendritic Cell Differentiation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.googleauthor | Kee Woong Kwon | - |
dc.contributor.googleauthor | So Jeong Kim | - |
dc.contributor.googleauthor | Hongmin Kim | - |
dc.contributor.googleauthor | Woo Sik Kim | - |
dc.contributor.googleauthor | Soon Myung Kang | - |
dc.contributor.googleauthor | Eunsol Choi | - |
dc.contributor.googleauthor | Sang-Jun Ha | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.contributor.googleauthor | Sung Jae Shin | - |
dc.identifier.doi | 10.7150/ijbs.25743 | - |
dc.contributor.localId | A02114 | - |
dc.contributor.localId | A02604 | - |
dc.relation.journalcode | J01091 | - |
dc.identifier.eissn | 1449-2288 | - |
dc.identifier.pmid | 30745835 | - |
dc.subject.keyword | B220 | - |
dc.subject.keyword | Dendritic cells | - |
dc.subject.keyword | IFN-γ | - |
dc.subject.keyword | IL-15 | - |
dc.subject.keyword | Mycobacterium tuberculosis | - |
dc.contributor.alternativeName | Shin, Sung Jae | - |
dc.contributor.affiliatedAuthor | 신성재 | - |
dc.contributor.affiliatedAuthor | 윤주헌 | - |
dc.citation.volume | 15 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 464 | - |
dc.citation.endPage | 480 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, Vol.15(2) : 464-480, 2019 | - |
dc.identifier.rimsid | 58351 | - |
dc.type.rims | ART | - |
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