291 494

Cited 0 times in

Transgenic overexpression of human LY6K in mice suppresses mature T cell development in the thymus

Authors
 Dasom Son  ;  Hyun-Kyung Kong  ;  Yesol Kim  ;  Min-Ji Song  ;  Hyong Pyo Kim  ;  Han Woong Lee  ;  Jong Hoon Park 
Citation
 ONCOLOGY LETTERS, Vol.17(1) : 379-387, 2019 
Journal Title
ONCOLOGY LETTERS
ISSN
 1792-1074 
Issue Date
2019
Keywords
T cell development ; breast cancer ; lymphocyte antigen 6 family member K ; lymphocytes ; spleen ; thymus ; transgenic mouse model
Abstract
Lymphocyte antigen 6 family member K (LY6K) is upregulated in a number of types of cancer and promotes tumor cell proliferation and metastasis. In addition, LY6K is involved in tamoxifen resistance in breast cancer. However, the in vivo molecular mechanism of LY6K has not yet been investigated. In the present study, transgenic mice overexpressing human LY6K (hLY6K) were generated using the pMAMneo vector, and the effect of LY6K upregulation in vivo was investigated. A total of 4 transgenic mice were generated, and the gene copy number was examined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). RT-qPCR demonstrated that mRNA of hLY6K was overexpressed in the thymus and spleen of the transgenic mice compared with wild-type mice. Flow cytometric analysis demonstrated that the proportions of B and T cells in the spleen were similar in wild-type and transgenic mice; however, the proportion of thymic mature T cells decreased in the transgenic mice, while there was an increase in the proportion of naïve T cells. These findings suggest that the overexpression of LY6K suppresses T cell development, and that LY6K is a potential therapeutic target for cancer.
Files in This Item:
T201900342.pdf Download
DOI
10.3892/ol.2018.9548
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyoung Pyo(김형표) ORCID logo https://orcid.org/0000-0003-1441-8822
Song, Min Ji(송민지)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/167544
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links