Cited 13 times in
Association Between Impairment of DNA Double Strand Break Repair and Decreased Ovarian Reserve in Patients With Endometriosis
DC Field | Value | Language |
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dc.contributor.author | 김현수 | - |
dc.contributor.author | 박주현 | - |
dc.contributor.author | 서석교 | - |
dc.contributor.author | 성학준 | - |
dc.contributor.author | 윤보현 | - |
dc.contributor.author | 이병석 | - |
dc.contributor.author | 이재훈 | - |
dc.contributor.author | 조시현 | - |
dc.contributor.author | 최영식 | - |
dc.contributor.author | 윤정기 | - |
dc.date.accessioned | 2019-02-14T01:52:12Z | - |
dc.date.available | 2019-02-14T01:52:12Z | - |
dc.date.issued | 2018 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/167266 | - |
dc.description.abstract | Background: Repair of DNA double strand break (DSB) is an important mechanism for maintaining genetic stability during a DNA damage event. Although, a growing body of recent evidence suggests that DNA DSBs and related repair mechanisms may be important in ovarian aging and in various cancers, there are few reports in endometriosis. We, therefore, examined expression levels of genes pertaining to DNA DSB repair in patients with endometriosis to assess the potential effects on ovarian reserves. Materials and methods: A total of 69 women undergoing laparoscopic surgery for endometriosis and other benign conditions was included; endometriosis group (n = 38) vs. controls (n = 31). DNA DSBs in endometrial and ovarian tissues of both groups were compared via immunohistochemistry, aimed at γ-H2AX expression. To gauge genotoxin-induced DNA DSBs in endometrial stromal cells, γ-H2AX expression was determined by western blot after H2O2 treatment of cultured endometrial stromal cells (endometriosis group and controls) and Ishikawa cell-line cultures. Endometrial and ovarian tissue levels of BRCA1, BRCA2, Rad51, and ATM (ataxia-telangiectasia mutated) mRNA expression were also compared. Correlations between expression levels of genes of interest and serum anti-müllerian hormone (AMH) levels were assessed as well. Results: Expression of γ-H2AX in immunostained endometrial and ovarian tissue preparations was greater in the endometriosis group, compared with controls. After H2O2 treatment, γ-H2AX expression levels were also significantly greater in cultured stromal cells of the endometriosis group and in the Ishikawa cell line than in controls. Endometrial expression of BRCA1 and Rad51 mRNA proved significantly lower in the endometriosis group (vs. controls), as did ovarian expression of BRCA1 and BRCA2 mRNA. Serum AMH concentration showed a significant correlation with ovarian BRCA1 mRNA expression in women with endometriosis (p = 0.03). Conclusions: In women with endometriosis, expression levels of various genes implicated in DSB repair are decreased and ovarian BRCA1 expression correlates with. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Frontiers Research | - |
dc.relation.isPartOf | FRONTIERS IN ENDOCRINOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Association Between Impairment of DNA Double Strand Break Repair and Decreased Ovarian Reserve in Patients With Endometriosis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pathology (병리학교실) | - |
dc.contributor.googleauthor | Young Sik Choi | - |
dc.contributor.googleauthor | Ji Hyun Park | - |
dc.contributor.googleauthor | Jae Hoon Lee | - |
dc.contributor.googleauthor | Jeong-Kee Yoon | - |
dc.contributor.googleauthor | Bo Hyon Yun | - |
dc.contributor.googleauthor | Joo Hyun Park | - |
dc.contributor.googleauthor | Seok Kyo Seo | - |
dc.contributor.googleauthor | Hak-Joon Sung | - |
dc.contributor.googleauthor | Hyun-Soo Kim | - |
dc.contributor.googleauthor | SiHyun Cho | - |
dc.contributor.googleauthor | Byung Seok Lee | - |
dc.identifier.doi | 10.3389/fendo.2018.00772 | - |
dc.contributor.localId | A01114 | - |
dc.contributor.localId | A01668 | - |
dc.contributor.localId | A01888 | - |
dc.contributor.localId | A01958 | - |
dc.contributor.localId | A02555 | - |
dc.contributor.localId | A02795 | - |
dc.contributor.localId | A04636 | - |
dc.contributor.localId | A03846 | - |
dc.contributor.localId | A04114 | - |
dc.relation.journalcode | J03412 | - |
dc.identifier.eissn | 1664-2392 | - |
dc.identifier.pmid | 30622513 | - |
dc.subject.keyword | BRCA1 gene | - |
dc.subject.keyword | double stranded DNA break | - |
dc.subject.keyword | endometriosis | - |
dc.subject.keyword | gamma-H2AX | - |
dc.subject.keyword | ovarian reserve | - |
dc.contributor.alternativeName | Kim, Hyun-Soo | - |
dc.contributor.affiliatedAuthor | 김현수 | - |
dc.contributor.affiliatedAuthor | 박주현 | - |
dc.contributor.affiliatedAuthor | 서석교 | - |
dc.contributor.affiliatedAuthor | 성학준 | - |
dc.contributor.affiliatedAuthor | 윤보현 | - |
dc.contributor.affiliatedAuthor | 이병석 | - |
dc.contributor.affiliatedAuthor | 이재훈 | - |
dc.contributor.affiliatedAuthor | 조시현 | - |
dc.contributor.affiliatedAuthor | 최영식 | - |
dc.citation.volume | 9 | - |
dc.citation.startPage | 772 | - |
dc.identifier.bibliographicCitation | FRONTIERS IN ENDOCRINOLOGY, Vol.9 : 772, 2018 | - |
dc.identifier.rimsid | 61470 | - |
dc.type.rims | ART | - |
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