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Human Papillomavirus 16 Oncoproteins Downregulate the Expression of miR-148a-3p, miR-190a-5p, and miR-199b-5p in Cervical Cancer

DC Field Value Language
dc.contributor.author김재훈-
dc.contributor.author김현숙-
dc.contributor.author이재면-
dc.date.accessioned2019-01-15T17:00:53Z-
dc.date.available2019-01-15T17:00:53Z-
dc.date.issued2018-
dc.identifier.issn2314-6133-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/166760-
dc.description.abstractAlmost all cervical cancers are associated with human papillomavirus (HPV); however, the majority of women infected with this virus do not develop cervical cancer. Therefore, new markers are needed for reliable screening of cervical cancer, especially in relation to HPV infection. We aimed to identify potential microRNAs that may serve as diagnostic markers for cervical cancer development in high-risk HPV-positive patients. We evaluated the microRNA expression profiles in 12 cervical tissues using the hybridization method and verified them by quantitative polymerase chain reaction (qPCR). Finally, we evaluated the effects of HPV16 oncoproteins on the expression of selected microRNAs using cervical cancer cells (CaSki and SiHa) and RNA interference. With the hybridization method, eight microRNAs (miR-9-5p, miR-136-5p, miR-148a-3p, miR-190a-5p, miR-199b-5p, miR-382-5p, miR-597-5p, and miR-655-3p) were found to be expressed differently in the HPV16-positive cervical cancer group and HPV16-positive normal group (fold change ≥ 2). The results of qPCR showed that miR-148a-3p, miR-190a-5p, miR-199b-5p, and miR-655-3p levels significantly decreased in the cancer group compared with the normal group. Upon silencing of HPV16 E5 and E6/E7, miR-148a-3p levels increased in both cell lines. Silencing of E6/E7 in SiHa cells led to the increase in miR-199b-5p and miR-190a-5p levels. Three HPV16 oncoproteins (E5, E6, and E7) downregulate miR-148a-3p, while E6/E7 inhibit miR-199b-5p and miR-190a-5p expression in cervical carcinoma. The three microRNAs, miR-148a-3p, miR-199b-5p, and miR-190a-5p, may be novel diagnostic biomarkers for cervical cancer development in high-risk HPV-positive patients.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherHindawi Pub. Co.-
dc.relation.isPartOfBIOMED RESEARCH INTERNATIONAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleHuman Papillomavirus 16 Oncoproteins Downregulate the Expression of miR-148a-3p, miR-190a-5p, and miR-199b-5p in Cervical Cancer-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Obstetrics and Gynecology (산부인과학교실)-
dc.contributor.googleauthorMi-Soon Han-
dc.contributor.googleauthorJae Myun Lee-
dc.contributor.googleauthorSoo-Nyung Kim-
dc.contributor.googleauthorJae-Hoon Kim-
dc.contributor.googleauthorHyon-Suk Kim-
dc.identifier.doi10.1155/2018/1942867-
dc.contributor.localIdA00876-
dc.contributor.localIdA01117-
dc.contributor.localIdA01117-
dc.contributor.localIdA03071-
dc.contributor.localIdA03071-
dc.relation.journalcodeJ00315-
dc.identifier.eissn2314-6141-
dc.contributor.alternativeNameKim, Jae Hoon-
dc.contributor.affiliatedAuthor김재훈-
dc.contributor.affiliatedAuthor김현숙-
dc.contributor.affiliatedAuthor김현숙-
dc.contributor.affiliatedAuthor이재면-
dc.contributor.affiliatedAuthor이재면-
dc.citation.volume2018-
dc.citation.startPage1942867-
dc.identifier.bibliographicCitationBIOMED RESEARCH INTERNATIONAL, Vol.2018 : 1942867, 2018-
dc.identifier.rimsid58028-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers

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