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Pulmonary preservation studies: effects on endothelial function and pulmonary adenine nucleotides

Authors
 Paik, Hyo Chae  ;  Hoffmann, Steven C.  ;  Egan, Thomas M. 
Citation
 Transplantation, Vol.75(4) : 439-444, 2003 
Journal Title
 Transplantation 
ISSN
 0041-1337 
Issue Date
2003
MeSH
Adenine Nucleotides/metabolism* ; Adenosine/pharmacology ; Adenosine Diphosphate/metabolism ; Adenosine Monophosphate/metabolism ; Adenosine Triphosphate/metabolism ; Allopurinol/pharmacology ; Animals ; Capillary Permeability/drug effects ; Cyclic AMP/metabolism ; Dextrans/pharmacology ; Endothelium, Vascular/metabolism ; Glucose/pharmacology ; Glutathione/pharmacology ; Hypertonic Solutions/pharmacology* ; Insulin/pharmacology ; Lung/drug effects* ; Lung/metabolism ; Lung/pathology ; Lung Transplantation* ; Male ; Organ Preservation Solutions/pharmacology* ; Organ Size ; Raffinose/pharmacology ; Rats ; Rats, Sprague-Dawley ; Water
Abstract
BACKGROUND: Lung transplantation is an effective therapy plagued by a high incidence of early graft dysfunction, in part because of reperfusion injury. The optimal preservation solution for lung transplantation is unknown. We performed experiments using an isolated perfused rat lung model to test the effect of lung preservation with three solutions commonly used in clinical practice. METHODS: Lungs were retrieved from Sprague-Dawley rats and flushed with one of three solutions: modified Euro-Collins (MEC), University of Wisconsin (UW), or low potassium dextran and glucose (LPDG), then stored cold for varying periods before reperfusion with Earle's balanced salt solution using the isolated perfused rat lung model. Outcome measures were capillary filtration coefficient (Kfc), wet-to-dry weight ratio, and lung tissue levels of adenine nucleotides and cyclic AMP. RESULTS: All lungs functioned well after 4 hr of storage. By 6 hr, UW-flushed lungs had a lower Kfc than LPDG-flushed lungs. After 8 hr of storage, only UW-flushed lungs had a measurable Kfc. Adenine nucleotide levels were higher in UW-flushed lungs after prolonged storage. Cyclic AMP levels correlated with Kfc in all groups. CONCLUSIONS: Early changes in endothelial permeability seemed to be better attenuated in lungs flushed with UW compared with LPDG or MEC; this was associated with higher amounts of adenine nucleotides. MEC-flushed lungs failed earlier than LPDG-flushed or UW-flushed lungs. The content of the solution may be more important for lung preservation than whether the ionic composition is intracellular or extracellular.
Full Text
https://oce.ovid.com/article/00007890-200302270-00004/HTML
DOI
10.1097/01.TP.0000045685.09601.9E
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
Yonsei Authors
Paik, Hyo Chae(백효채) ORCID logo https://orcid.org/0000-0001-9309-8235
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/166180
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