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Conserved Noncoding Sequences Boost ADR1 and SP1 Regulated Human Swiprosin-1 Promoter Activity
DC Field | Value | Language |
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dc.contributor.author | 안성용 | - |
dc.contributor.author | 현영민 | - |
dc.date.accessioned | 2018-11-26T16:55:53Z | - |
dc.date.available | 2018-11-26T16:55:53Z | - |
dc.date.issued | 2018 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/165867 | - |
dc.description.abstract | Swiprosin-1 is expressed in various types of cells or tissues of different species. To investigate the mechanisms underlying Swiprosin-1 expression pattern, we analyzed the promoter activity of 2-kilobase genomic sequences located at 5' flanking region of the Swiprosin-1 gene. The -2000/+41 bp of 5' flanking untranslated promoter region of Swiprosin-1 gene was constitutively transactivated without significant effect of PMA, A23187, or PMA/A23187 stimulation in Jurkat T cells. Further, we identified 5' deletant of proximal promoter region (-100/+41 to -70/+41) plays a pivotal role in activating the Swiprosin-1 gene in Jurkat T cells. Our studies also verified that ADR1 and Sp1 transcription factors were located between -70 and -100 locus of 5' flanking proximal promoter region, which is critical for the Swiprosin-1 promoter activity. ADR1 and Sp1 were shown to bind the regions of -82, -79, -76, -73 and -70 and; -79, -78 and -77, respectively, within the proximal promoter region of Swiprosin-1. Finally conserved noncoding sequences (CNS) -1, -2 and -3 were located between the exon 1 and exon 2 of Swiprosin-1 gene and synergistically transactivated the Swiprosin-1 promoter. In summary, Swiprosin-1 was constitutively expressed in Jurkat T cells by the coordinate action of ADR1 and SP1 transcription factors at the transcriptional level and CNS further boost the proximal region of Swiprosin-1 promoter activity. Our findings provide novel insights that the transcriptional regulation of Swiprosin-1 by targeting ADR1 and Sp1 binding sites may be helpful in exploring novel therapeutic strategies for advanced immune or other disorders. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Conserved Noncoding Sequences Boost ADR1 and SP1 Regulated Human Swiprosin-1 Promoter Activity | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anatomy (해부학교실) | - |
dc.contributor.googleauthor | Ramesh P. Thylur | - |
dc.contributor.googleauthor | Sung Yong Ahn | - |
dc.contributor.googleauthor | Eunhea Jung | - |
dc.contributor.googleauthor | Chang-Duk Jun | - |
dc.contributor.googleauthor | Young-Min Hyun | - |
dc.identifier.doi | 10.1038/s41598-018-34802-z | - |
dc.contributor.localId | A05145 | - |
dc.contributor.localId | A04814 | - |
dc.relation.journalcode | J02646 | - |
dc.identifier.eissn | 2045-2322 | - |
dc.identifier.pmid | 30405162 | - |
dc.contributor.alternativeName | Ahn, Sung Yong | - |
dc.contributor.affiliatedAuthor | 안성용 | - |
dc.contributor.affiliatedAuthor | 현영민 | - |
dc.citation.volume | 8 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 16481 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, Vol.8(1) : 16481, 2018 | - |
dc.identifier.rimsid | 59260 | - |
dc.type.rims | ART | - |
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