0 189

Cited 0 times in

Overexpression of WNK1 in POMC-expressing neurons reduces weigh gain via WNK4-mediated degradation of Kir6.2.

DC FieldValueLanguage
dc.contributor.author김주영-
dc.contributor.author이민구-
dc.date.accessioned2018-11-08T16:40:40Z-
dc.date.available2018-11-08T16:40:40Z-
dc.date.issued2018-
dc.identifier.issn0300-8177-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/165118-
dc.description.abstract"With no lysine" (WNK) kinases have been shown to regulate various ion transporters in various tissues, but studies on the function of WNK kinases in the brain have been limited. In this study, we discovered that WNK1 and WNK4 in POMC-expressing neuronal cells in WNK1 overexpressed transgenic mice (WNK1 TG) decrease appetite via degradation of Kir6.2. Weight gain after 20 weeks of age was delayed in WNK1 TG mice as a result of reduced food intake. Expression of WNK1 and proopiomelanocortin (POMC) was higher in POMC-expressing neurons in the hypothalamus of WNK1 TG mice than in WT mice. Immunostaining of serial sections of the hypothalamus revealed that POMC-expressing neurons were smaller in WNK1 TG mice than in WT mice. In addition, expression of Kir6.2 was significantly reduced in WNK1 TG mice. Overexpression and knockdown of WNK4 demonstrated that WNK4 regulates protein expression of Kir6.2 via protein-protein interaction. Accordingly, reduced age-dependent weight gain of WNK1 TG mice seems to be related with the decreased Kir6.2 expression via WNK1- and WNK4-regulated protein stability of Kir6.2.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherKluwer Academic-
dc.relation.isPartOfMOLECULAR AND CELLULAR BIOCHEMISTRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAging/genetics-
dc.subject.MESHAging/metabolism-
dc.subject.MESHAnimals-
dc.subject.MESHGene Expression Regulation*-
dc.subject.MESHHEK293 Cells-
dc.subject.MESHHumans-
dc.subject.MESHHypothalamus/cytology-
dc.subject.MESHHypothalamus/metabolism*-
dc.subject.MESHKATP Channels/genetics-
dc.subject.MESHKATP Channels/metabolism*-
dc.subject.MESHMice-
dc.subject.MESHMice, Transgenic-
dc.subject.MESHNeurons/cytology-
dc.subject.MESHNeurons/metabolism*-
dc.subject.MESHPro-Opiomelanocortin/biosynthesis*-
dc.subject.MESHPro-Opiomelanocortin/genetics-
dc.subject.MESHProtein Stability-
dc.subject.MESHProtein-Serine-Threonine Kinases/biosynthesis*-
dc.subject.MESHProtein-Serine-Threonine Kinases/genetics-
dc.subject.MESHProteolysis*-
dc.subject.MESHRats-
dc.subject.MESHWNK Lysine-Deficient Protein Kinase 1/biosynthesis*-
dc.subject.MESHWNK Lysine-Deficient Protein Kinase 1/genetics-
dc.titleOverexpression of WNK1 in POMC-expressing neurons reduces weigh gain via WNK4-mediated degradation of Kir6.2.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학교실)-
dc.contributor.googleauthorWoo Young Chung-
dc.contributor.googleauthorJung Woo Han-
dc.contributor.googleauthorWoon Heo-
dc.contributor.googleauthorMin Goo Lee-
dc.contributor.googleauthorJoo Young Kim-
dc.identifier.doi10.1007/s11010-018-3301-4-
dc.contributor.localIdA00942-
dc.contributor.localIdA00942-
dc.contributor.localIdA02781-
dc.relation.journalcodeJ02242-
dc.identifier.eissn1573-4919-
dc.identifier.pmid29392534-
dc.identifier.urlhttps://link.springer.com/article/10.1007%2Fs11010-018-3301-4-
dc.subject.keywordAppetite-
dc.subject.keywordKir6.2-
dc.subject.keywordPOMC-expressing neuron-
dc.subject.keywordProtein degradation-
dc.subject.keywordTransgenic mice-
dc.subject.keywordWNK1-
dc.subject.keywordWNK4-
dc.contributor.alternativeNameKim, Joo Young-
dc.contributor.alternativeNameLee, Min Goo-
dc.contributor.affiliatedAuthor김주영-
dc.contributor.affiliatedAuthor이민구-
dc.citation.volume447-
dc.citation.number1~2-
dc.citation.startPage165-
dc.citation.endPage174-
dc.identifier.bibliographicCitationMOLECULAR AND CELLULAR BIOCHEMISTRY, Vol.447(1~2) : 165-174, 2018-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.