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Molecular Characterization of Pseudomonas putida Group Isolates Carrying blaVIM-2 Disseminated in a University Hospital in Korea

Authors
 Jun Sung Hong  ;  Eun-Jeong Yoon  ;  Wonkeun Song  ;  Yu Bin Seo  ;  Saeam Shin  ;  Min-Jeong Park  ;  Seok Hoon Jeong  ;  Kyungwon Lee 
Citation
 Microbial Drug Resistance, Vol.24(5) : 627-634, 2018 
Journal Title
 Microbial Drug Resistance 
ISSN
 1076-6294 
Issue Date
2018
Keywords
Pseudomonas monteilii ; Pseudomonas putida ; blaVIM-2 ; class I integron ; horizontal transfer
Abstract
Pseudomonas putida group are Gram-negative bacilli with polar flagellation, which are ubiquitous in the environment, although they are rarely involved in human infections. The aim of this study was to identify the dissemination of VIM-2-producing P. putida group in clinical isolates from a hospital in Korea. Thirteen strains were collected from 2014 to 2015 for the study. The isolates were recovered from urine cultures of both inpatients and outpatients at the hospital. Minimum inhibitory concentrations of antibiotics were determined by Etest. Carbapenemase genes were amplified by polymerase chain reaction and sequenced. Pulsed-field gel electrophoresis was performed for strain typing. Whole-genome sequencing was carried out randomly for two strains chosen from each year of the study to analyze the plasmid structure carrying the blaVIM-2 genes. The 13 isolates carried nine different class I integrons harboring VIM-2 and were resistant to meropenem and imipenem (minimum inhibitory concentrations, >/=32 mug/ml), thus exhibiting a multidrug-resistant phenotype. The blaVIM-2 gene was located on a plasmid in seven of the isolates and on the chromosome in six isolates. Each case of the blaVIM-2 gene was disseminated by clonal spread, horizontal transfer, and was mostly an occasional occurrence. In this study, we demonstrated that multidrug-resistant P. putida group carrying VIM-2 has reemerged in human specimens in Korea.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/162559
Full Text
https://www.liebertpub.com/doi/10.1089/mdr.2017.0257
DOI
10.1089/mdr.2017.0257
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실)
Yonsei Authors
이경원(Lee, Kyungwon) ORCID logo https://orcid.org/0000-0003-3788-2134
정석훈(Jeong, Seok Hoon) ORCID logo https://orcid.org/0000-0001-9290-897X
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