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Extended Culture of Bone Marrow with Granulocyte Macrophage-Colony Stimulating Factor Generates Immunosuppressive Cells

Authors
 Hye Young Na  ;  Moah Sohn  ;  Seul Hye Ryu  ;  Wanho Choi  ;  Hyunju In  ;  Hyun Soo Shin  ;  Chae Gyu Park 
Citation
 Immune Network, Vol.18(2) : e16, 2018 
Journal Title
 Immune Network 
ISSN
 1598-2629 
Issue Date
2018
Keywords
Bone marrow ; Dendritic cells ; Granulocyte-macrophage colony-stimulating factor ; Immunosuppression ; mixed Lymphocyte culture test
Abstract
Bone marrow-derived dendritic cells (BM-DCs) are generated from bone marrow (BM) cells cultured with granulocyte macrophage-colony stimulating factor (GM-CSF) for a week. In this study we investigated the effect of duration on the BM culture with GM-CSF. Within several months, the cells in the BM culture gradually expressed homogeneous levels of CD11c and major histocompatibility complex II on surface, and they became unable to stimulate allogeneic naive T cells in mixed lymphocyte reaction (MLR). In addition, when the BM culture were sustained for 32 wk or longer, the BM cells acquired ability to suppress the proliferation of allogeneic T cells in MLR as well as the response of ovalbumin-specific OT-I transgenic T cells in antigen-dependent manner. We found that, except for programmed death-ligand 1, most cell surface molecules were expressed lower in the BM cells cultured with GM-CSF for the extended duration. These results indicate that BM cells in the extended culture with GM-CSF undergo 2 distinct steps of functional change; first, they lose the immunostimulatory capacity; and next, they gain the immunosuppressive ability.
Files in This Item:
T201801899.pdf Download
DOI
10.4110/in.2018.18.e16
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Na, Hye Young(나혜영) ORCID logo https://orcid.org/0000-0002-2886-9926
Park, Chae Gyu(박채규) ORCID logo https://orcid.org/0000-0003-1906-1308
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/162547
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