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O-GlcNAcylation of the Tumor Suppressor FOXO3 Triggers Aberrant Cancer Cell Growth

Authors
 Heon Shin  ;  Hyun-Jeong Cha  ;  Keun Na  ;  Min Jung Lee  ;  Jin-Young Cho  ;  Chae-Yeon Kim  ;  Eun Kyung Kim  ;  Chang Moo Kang  ;  Hoguen Kim  ;  Young-Ki Paik 
Citation
 Cancer Research, Vol.78(5) : 1214-1224, 2018 
Journal Title
 Cancer Research 
ISSN
 0008-5472 
Issue Date
2018
Abstract
Posttranslational modifications of tumor suppressors can induce abnormal cell growth. Here, we identify site-specific O-GlcNAcylation as a critical block of FOXO3 that may abrogate a part of the p53 pathway, resulting in aberrant cancer cell growth. Of seven O-GlcNAcylation sites identified within the FOXO3 transactivation domain, we found that changes in O-GlcNAcylation at Ser284 modulated p21-mediated cancer cell growth. Overexpression of either O-GlcNAcylated FOXO3 (FOX-OV) or a Ser-to-Ala mutant (S284A) in PANC-1 cells indicated that S284 O-GlcNAc acts as a critical block of the FOXO tumor suppressor and induces proliferation in PANC-1 cancer cells by stimulating the MDM2-p53-p21 axis. Furthermore, S284A mutant cells lacking S284 O-GlcNAc and FOX-OV cells exhibited opposing MDM2-p53-p21 axis expression patterns at both the mRNA and protein levels. Thus, our study provides evidence to support a role for S284 O-GlcNAc as a critical block of FOXO3 to induce subsequent cancer cell growth via abrogation of the p53 regulatory circuit.Significance: These findings highlight a posttranslational mechanism for indirect abrogation of the p53 pathway, one that may occur with some frequency in human cancer cells. Cancer Res; 78(5); 1214-24. (c)2018 AACR.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/162452
Full Text
http://cancerres.aacrjournals.org/content/78/5/1214
DOI
10.1158/0008-5472.can-17-3512
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실)
Yonsei Authors
강창무(Kang, Chang Moo) ORCID logo https://orcid.org/0000-0002-5382-4658
김은경(Kim, Eun Kyung)
김호근(Kim, Ho Keun)
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