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Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue

Authors
 Jiwon Woo  ;  Eunice Yoojin Lee  ;  Hyo-Suk Park  ;  Jeong Yoon Park  ;  Yong Eun Cho 
Citation
 Journal of Visualized Experiments : JoVE, Vol.0(135), 2018 
Journal Title
 Journal of Visualized Experiments : JoVE 
Issue Date
2018
Abstract
Since the development of CLARITY, a bioelectrochemical clearing technique that allows for three-dimensional phenotype mapping within transparent tissues, a multitude of novel clearing methodologies including CUBIC (clear, unobstructed brain imaging cocktails and computational analysis), SWITCH (system-wide control of interaction time and kinetics of chemicals), MAP (magnified analysis of the proteome), and PACT (passive clarity technique), have been established to further expand the existing toolkit for the microscopic analysis of biological tissues. The present study aims to improve upon and optimize the original PACT procedure for an array of intact rodent tissues, including the whole central nervous system (CNS), kidneys, spleen, and whole mouse embryos. Termed psPACT (process-separate PACT) and mPACT (modified PACT), these novel techniques provide highly efficacious means of mapping cell circuitry and visualizing subcellular structures in intact normal and pathological tissues. In the following protocol, we provide a detailed, step-by-step outline on how to achieve maximal tissue clearance with minimal invasion of their structural integrity via psPACT and mPACT.
Full Text
https://www.jove.com/video/57123/novel-passive-clearing-methods-for-rapid-production-optical
DOI
10.3791/57123
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
박정윤(Park, Jeong Yoon) ORCID logo https://orcid.org/0000-0002-3728-7784
조용은(Cho, Yong Eun) ORCID logo https://orcid.org/0000-0001-9815-2720
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/162440
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