Composite scaffold of micronized porcine cartilage/poly(lacticcoglycolic acid) enhances anti-inflammatory effect

Abstract

The main disadvantage of using poly(lacticcoglycolic acid) (PLGA), a typical synthetic polymer, as a biomaterial is that it induces inflammation. To overcome this disadvantage, we determined the ability of micronized porcine cartilage (MPC) for alleviating the inflammatory effects of a PLGA scaffold. MPC was analyzed by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and Fourier transform-infrared spectroscopy, and typical collagen components were confirmed. The MPC/PLGA scaffolds were fabricated using various concentrations of MPC and the compressive strength was evaluated to characterize its physical properties. Although the compressive strength decreased with increasing amounts of MPC, the roughness of the surface, assessed by scanning election microscopy, was considered to be suitable for facilitating cell attachment. Notably, in vitro experiments showed that the cell adhesion and proliferation rates increased as the MPC content increased. MPC further reduced gene expression levels of inflammatory cytokines and cellular reactive oxygen species, as determined by real time-polymerase chain reaction and fluorescence-activated cell sorting, respectively. In addition, in vivo experiments confirmed the interaction between tissues and the scaffolds. Overall, these results confirmed that the MPC/PLGA scaffold is superior to the PLGA scaffold in many respects and might be a suitable candidate for resolving the disadvantages of PLGA in tissue engineering applications.