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Olfactory marker protein regulates prolactin secretion and production by modulating Ca(2+) and TRH signaling in lactotrophs

 Chan Woo Kang  ;  Ye Eon Han  ;  Mi Kyung Lee  ;  Yoon Hee Cho  ;  NaNa Kang  ;  JaeHyung Koo  ;  Cheol Ryong Ku  ;  Eun Jig Lee 
 Experimental and Molecular Medicine, Vol.50(4) : 15, 2018 
Journal Title
 Experimental and Molecular Medicine 
Issue Date
Olfactory marker protein (OMP) is a marker of olfactory receptor-mediated chemoreception, even outside the olfactory system. Here, we report that OMP expression in the pituitary gland plays a role in basal and thyrotropin-releasing hormone (TRH)-induced prolactin (PRL) production and secretion. We found that OMP was expressed in human and rodent pituitary glands, especially in PRL-secreting lactotrophs. OMP knockdown in GH4 rat pituitary cells increased PRL production and secretion via extracellular signal-regulated kinase (ERK)1/2 signaling. Real-time PCR analysis and the Ca(2+) influx assay revealed that OMP was critical for TRH-induced PRL secretion. OMP-knockout mice showed lower fertility than control mice, which was associated with increased basal PRL production via activation of ERK1/2 signaling and reduced TRH-induced PRL secretion. However, both in vitro and in vivo results indicated that OMP was only required for hormone production and secretion because ERK1/2 activation failed to stimulate cell proliferation. Additionally, patients with prolactinoma lacked OMP expression in tumor tissues with hyperactivated ERK1/2 signaling. These findings indicate that OMP plays a role in PRL production and secretion in lactotrophs through the modulation of Ca(2+) and TRH signaling.
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1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Institute of Endocrinology (내분비연구소)
Yonsei Authors
구철룡(Ku, Cheol Ryong) ORCID logo https://orcid.org/0000-0001-8693-9630
이은직(Lee, Eun Jig) ORCID logo https://orcid.org/0000-0002-9876-8370
조윤희(Cho, Yoon Hee)
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