224 217

Cited 6 times in

Stem cell properties of human clonal salivary gland stem cells are enhanced by three-dimensional priming culture in nanofibrous microwells

 Hyun-Soo Shin  ;  Songyi Lee  ;  Hye Jin Hong  ;  Young Chang Lim  ;  Won-Gun Koh  ;  Jae-Yol Lim 
 STEM CELL RESEARCH & THERAPY, Vol.9(1) : 74, 2018 
Journal Title
Issue Date
Micropatterned nanofibrous scaffolds ; Salivary glands ; Spheroid ; Wnt ; Xerostomia
BACKGROUND: Three-dimensional (3D) cultures recapitulate the microenvironment of tissue-resident stem cells and enable them to modulate their properties. We determined whether salivary gland-resident stem cells (SGSCs) are primed by a 3D spheroid culture prior to treating irradiation-induced salivary hypofunction using in-vitro coculture and in-vivo transplant models. METHODS: 3D spheroid-derived SGSCs (SGSCs(3D)) were obtained from 3D culture in microwells consisting of a nanofiber bottom and cell-repellent hydrogel walls, and were examined for salivary stem or epithelial gene/protein expression, differentiation potential, and paracrine secretory function compared with monolayer-cultured SGSCs (SGSCs(2D)) in vitro and in vivo. RESULTS: SGSCs(3D) expressed increased salivary stem cell markers (LGR5 and THY1) and pluripotency markers (POU5F1 and NANOG) compared with SGSCs(2D). Also, SGSCs(3D) exhibited enhanced potential to differentiate into salivary epithelial cells upon differentiation induction and increased paracrine secretion as compared to SGSCs(2D). Wnt signaling was activated by 3D spheroid formation in the microwells and suppression of the Wnt/beta-catenin pathway led to reduced stemness of SGSCs(3D). Enhanced radioprotective properties of SGSCs(3D) against radiation-induced salivary hypofunction was confirmed by an organotypic 3D coculture and in-vivo transplantation experiments. CONCLUSION: The 3D spheroid culture of SGSCs in nanofibrous microwells promotes stem cell properties via activation of Wnt signaling. This may contribute to SGSC priming prior to regenerative therapy to restore salivary hypofunction after radiotherapy.
Files in This Item:
T201800939.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Shin, Hyun Soo(신현수)
Lim, Jae Yol(임재열) ORCID logo https://orcid.org/0000-0002-9757-6414
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.