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The B cell death function of obinutuzumab-HDEL produced in plant (Nicotiana benthamiana L.) is equivalent to obinutuzumab produced in CHO cells
DC Field | Value | Language |
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dc.contributor.author | 김주영 | - |
dc.date.accessioned | 2018-08-28T16:57:04Z | - |
dc.date.available | 2018-08-28T16:57:04Z | - |
dc.date.issued | 2018 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/162102 | - |
dc.description.abstract | Plants have attracted attention as bio-drug production platforms because of their economical and safety benefits. The preliminary efficacy of ZMapp, a cocktail of antibodies produced in N. benthamiana (Nicotiana benthamiana L.), suggested plants may serve as a platform for antibody production. However, because the amino acid sequences of the Fab fragment are diverse and differences in post-transcriptional processes between animals and plants remain to be elucidated, it is necessary to confirm functional equivalence of plant-produced antibodies to the original antibody. In this study, Obinutuzumab, a third generation anti-CD20 antibody, was produced in N. benthamiana leaves (plant-obinutuzumab) and compared to the original antibody produced in glyco-engineered Chinese hamster ovary (CHO) cells (CHO-obinutuzumab). Two forms (with or without an HDEL tag) were generated and antibody yields were compared. The HDEL-tagged form was more highly expressed than the non-HDEL-tagged form which was cleaved in the N-terminus. To determine the equivalence in functions of the Fab region between the two forms, we compared the CD20 binding affinities and direct binding induced cell death of a CD20-positive B cells. Both forms showed similar CD20 binding affinities and direct cell death of B cell. The results suggested that plant-obinutuzumab was equivalent to CHO-obinutuzumab in CD20 binding, cell aggregation, and direct cell death via binding. Therefore, our findings suggest that Obinutuzumab is a promising biosimilar candidate that can be produced efficiently in plants. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Public Library of Science | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Monoclonal Antibodies, Humanized/*pharmacology | - |
dc.subject.MESH | Immunological/*pharmacology Antineoplastic Agents | - |
dc.subject.MESH | B-Lymphocytes/*cytology | - |
dc.subject.MESH | CHO Cells | - |
dc.subject.MESH | *Cell Death | - |
dc.subject.MESH | Cricetinae | - |
dc.subject.MESH | Cricetulus | - |
dc.subject.MESH | Epitopes/immunology | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | Plant Leaves/metabolism | - |
dc.subject.MESH | Tobacco/*genetics | - |
dc.title | The B cell death function of obinutuzumab-HDEL produced in plant (Nicotiana benthamiana L.) is equivalent to obinutuzumab produced in CHO cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Pharmacology | - |
dc.contributor.googleauthor | Jin Won Lee | - |
dc.contributor.googleauthor | Woon Heo | - |
dc.contributor.googleauthor | Jinu Lee | - |
dc.contributor.googleauthor | Narae Jin | - |
dc.contributor.googleauthor | Sei Mee Yoon | - |
dc.contributor.googleauthor | Ki Youl Park | - |
dc.contributor.googleauthor | Eun Yu Kim | - |
dc.contributor.googleauthor | Woo Taek Kim | - |
dc.contributor.googleauthor | Joo Young Kim | - |
dc.identifier.doi | 10.1371/journal.pone.0191075 | - |
dc.contributor.localId | A00942 | - |
dc.relation.journalcode | J02540 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.pmid | 29324849 | - |
dc.contributor.alternativeName | Kim, Joo Young | - |
dc.contributor.affiliatedAuthor | Kim, Joo Young | - |
dc.citation.volume | 13 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | e0191075 | - |
dc.identifier.bibliographicCitation | PLOS ONE, Vol.13(1) : e0191075, 2018 | - |
dc.identifier.rimsid | 59692 | - |
dc.type.rims | ART | - |
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