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Ezrin as a complementary marker in ocular toxicity assessment using a three-dimensional reconstructed human corneal-like epithelium model, EpiOcular

Authors
 Kyung Yuk Ko  ;  MiHye Hong  ;  Tae Sung Kim  ;  Ki Taek Nam  ;  GaYoung Lee  ;  Jung-Sun Yi  ;  Il Young Ahn  ;  Joo Hwan Kim  ;  Jong Kwon Lee 
Citation
 Journal of Pharmacological and Toxicological Methods, Vol.92 : 24-33, 2018 
Journal Title
 Journal of Pharmacological and Toxicological Methods 
ISSN
 1056-8719 
Issue Date
2018
Keywords
3D reconstructed human cornea-like epithelium model ; Complementary marker ; Eye irritation ; Ezrin
Abstract
INTRODUCTION: A variety of in vitro tests to replace the Draize test have been developed; however, there is no available method for assessing the full spectrum of Globally Harmonized System (GHS) categories. Human cornea-like three-dimensional (3D) reconstructed tissue models are the most promising in vitro systems. The objective of this study was to evaluate the ocular toxicity of 11 test substances using the EpiOcular model after performing proficiency tests. We further evaluated the effectiveness of ezrin staining as a complementary marker in histological analysis to overcome the limitation of eye irritation tests using 3D reconstructed human corneal epithelium models. METHODS: The assessment of ocular toxicity was performed by the suggested OECD TG 492 procedure. After treatment with proficiency test chemicals and 10 test substances, EpiOcular tissue models were stained with hematoxylin and eosin and ezrin, and the histological changes were observed by immunofluorescence microscopy. RESULTS: The ocular toxicity assessment of 10 test chemicals using the EpiOcular eye irritation test were in accordance with the UN GHS classification of test chemicals. Histological analysis of ezrin staining showed that the cell membranes of models treated with 10 out of 11 non-irritant chemicals were maintained, whereas those of models treated with 14 eye irritant substances resulted in the apparent translocation of ezrins from the cell membrane to the cytoplasm or nucleus by destruction of cell membrane. DISCUSSION: Ezrin may be used as a complementary marker to more accurately assess ocular toxicity using 3D reconstructed human cornea-like epithelium models.
Full Text
https://www.sciencedirect.com/science/article/pii/S1056871917301909
DOI
10.1016/j.vascn.2018.02.007
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Nam, Ki Taek(남기택)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/161963
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