0 42

Cited 1 times in

Inverted Quasi-Spherical Droplets on Polydopamine-TiO2 Substrates for Enhancing Gene Delivery.

Authors
 Seung‐Hyun Kim  ;  Mihyun Lee  ;  Mira Cho  ;  Il‐Sun Kim  ;  Kook In Park  ;  Haeshin Lee  ;  Jae‐Hyung Jang 
Citation
 Macromolecular Bioscience, Vol.17(11) : 1700148, 2017 
Journal Title
 Macromolecular Bioscience 
ISSN
 1616-5187 
Issue Date
2017
Keywords
adeno-associated virus ; gene delivery ; inverted droplet ; neural stem cells ; polydopamine
Abstract
Devising efficient gene delivery systems is crucial to enhancing the therapeutic efficacy of gene-cell therapy approaches. Herein, inverted quasi-spherical (iQS) droplet systems, which enhance gene delivery efficiencies by reducing the path lengths of gene vectors, mediating motions of vectors at early stages, and raising the contact frequencies of vectors with cells, are developed by adopting the principle of 3D hanging-drop cell culture. Micrometer-sized polydopamine (pDA) holes are created on superhydrophobic titanium isopropoxide (TiO2 )-coated substrates by physical scraping; droplets are loaded on the pDA holes, and inversion of the substrate generates iQS droplets with large contact angles. Both human neural stem cells (hNSCs) and adeno-associated viral vectors are simultaneously incorporated into the iQS droplets to assess gene delivery efficiencies. The steep angles of iQS droplets and enhanced cell/vector contact frequencies facilitate the viral association with hNSCs and enhancing cell-cell interactions, thereby significantly promoting gene delivery efficiencies. Even with reduced viral quantities/exposure times and cell numbers, the iQS droplet systems elicit sufficient gene expression (i.e., interleukin-10). The ability of the iQS droplet systems to maximize beneficial gene delivery effects with minimal materials (e.g., medium, cells, and vectors) should enable their extensive use as a platform for preparing genetically stimulated cellular therapeutics.
Full Text
https://onlinelibrary.wiley.com/doi/full/10.1002/mabi.201700148
DOI
10.1002/mabi.201700148
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
Yonsei Authors
김일선(Kim, Il-Sun) ORCID logo https://orcid.org/0000-0003-4033-4323
박국인(Park, Kook In) ORCID logo https://orcid.org/0000-0001-8499-9293
Export
RIS (EndNote)
XLS (Excel)
XML
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/161651
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse