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Practical Algorisms for PCR-RFLP-Based Genotyping of Echinococcus granulosus Sensu Lato

Authors
 Hye-Jin Kim  ;  Tae-Soon Yong  ;  Myeong Heon Shin  ;  Kyu-Jae Lee  ;  Gab-Man Park  ;  Uktamjon Suvonkulov  ;  Dmitriy Kovalenko  ;  Hak Sun Yu 
Citation
 Korean Journal of Parasitology, Vol.55(6) : 679-684, 2017 
Journal Title
 Korean Journal of Parasitology 
ISSN
 0023-4001 
Issue Date
2017
MeSH
Algorithms* ; Animals ; Animals, Domestic ; Cyclooxygenase 1/genetics ; Echinococcosis/parasitology* ; Echinococcosis/veterinary* ; Echinococcus granulosus/genetics* ; Echinococcus granulosus/isolation & purification ; Electron Transport Complex I/genetics ; Genotype* ; Genotyping Techniques/methods* ; Humans ; Polymerase Chain Reaction* ; Polymorphism, Restriction Fragment Length*
Keywords
Echinococcus granulosus ; cox1 ; genotyping ; nad1 ; practical algorism
Abstract
Echinococcus granulosus sensu lato (s.l.) is a causative agent of cystic echinococcosis or cystic hydatid disease in humans and domestic and wild animals. The disease is a serious health problem in countries associated with poverty and poor hygiene practices, particularly in livestock raising. We introduced a practical algorism for genotyping the parasite, which may be useful to many developing countries. To evaluate the efficiency of the algorism, we genotyped 3 unknown strains isolated from human patients. We found that unknowns 1 and 3 were included in G1, G2, and G3 genotypes group and unknown 2 was included in G4 genotype (Echinococcus equinus) according to the algorisms. We confirmed these results by sequencing the 3 unknown isolates cox1 and nad1 PCR products. In conclusion, these new algorisms are very fast genotype identification tools that are suitable for evaluating E. granulosus s.l. isolated from livestock or livestock holders, particularly in developing countries.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/161611
DOI
10.3347/kjp.2017.55.6.679
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실)
Yonsei Authors
신명헌(Shin, Myeong Heon) ; 용태순(Yong, Tai Soon)
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