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Heparin-functionalized polymer graft surface eluting MK2 inhibitory peptide to improve hemocompatibility and anti-neointimal activity

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dc.contributor.author박종철-
dc.contributor.author성학준-
dc.contributor.author선경미-
dc.date.accessioned2018-07-20T08:48:57Z-
dc.date.available2018-07-20T08:48:57Z-
dc.date.issued2017-
dc.identifier.issn0168-3659-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/161403-
dc.description.abstractThe leading cause of synthetic graft failure includes thrombotic occlusion and intimal hyperplasia at the site of vascular anastomosis. Herein, we report a co-immobilization strategy of heparin and potent anti-neointimal drug (Mitogen Activated Protein Kinase II inhibitory peptide; MK2i) by using a tyrosinase-catalyzed oxidative reaction for preventing thrombotic occlusion and neointimal formation of synthetic vascular grafts. The binding of heparin-tyramine polymer (HT) onto the polycarprolactone (PCL) surface enhanced blood compatibility with significantly reduced protein absorption (64.7% decrease) and platelet adhesion (85.6% decrease) compared to bare PCL surface. When loading MK2i, 1) the HT depot surface gained high MK2i-loading efficiency through charge-charge interaction, and 2) this depot platform enabled long-term, controlled release over 4weeks (92-272μg/mL of MK2i). The released MK2i showed significant inhibitory effects on VSMC migration through down-regulated phosphorylation of target proteins (HSP27 and CREB) associated with intimal hyperplasia. In addition, it was found that the released MK2i infiltrated into the tissue with a cumulative manner in ex vivo human saphenous vein (HSV) model. This present study demonstrates that enzymatically HT-coated surface modification is an effective strategy to induce long-term MK2i release as well as hemocompatibility, thereby improving anti-neointimal activity of synthetic vascular grafts.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier Science Publishers-
dc.relation.isPartOfJOURNAL OF CONTROLLED RELEASE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleHeparin-functionalized polymer graft surface eluting MK2 inhibitory peptide to improve hemocompatibility and anti-neointimal activity-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Medical Engineering-
dc.contributor.googleauthorYunki Lee-
dc.contributor.googleauthorPhuong Le Thi-
dc.contributor.googleauthorGyeung Mi Seon-
dc.contributor.googleauthorSeung Bae Ryu-
dc.contributor.googleauthorColleen M. Brophy-
dc.contributor.googleauthorYongTae Kim-
dc.contributor.googleauthorJong-Chul Park-
dc.contributor.googleauthorKi Dong Park-
dc.contributor.googleauthorJoyce Cheung-Flynn-
dc.contributor.googleauthorHak-Joon Sung-
dc.identifier.doi10.1016/j.jconrel.2017.10.002-
dc.contributor.localIdA01662-
dc.contributor.localIdA01958-
dc.relation.journalcodeJ01352-
dc.identifier.eissn1873-4995-
dc.identifier.pmid28987880-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0168365917308891-
dc.subject.keywordAnti-thrombosis-
dc.subject.keywordHeparin-
dc.subject.keywordIntimal hyperplasia-
dc.subject.keywordMK2 inhibitory peptide-
dc.subject.keywordSynthetic vascular grafts-
dc.subject.keywordTyrosinase-triggered surface functionalization-
dc.contributor.alternativeNamePark, Jong Chul-
dc.contributor.alternativeNameSung, Hak-Joon-
dc.contributor.affiliatedAuthorPark, Jong Chul-
dc.contributor.affiliatedAuthorSung, Hak-Joon-
dc.citation.volume266-
dc.citation.startPage321-
dc.citation.endPage330-
dc.identifier.bibliographicCitationJOURNAL OF CONTROLLED RELEASE, Vol.266 : 321-330, 2017-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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