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The Flavonoid Glabridin Induces OCT4 to Enhance Osteogenetic Potential in Mesenchymal Stem Cells

Authors
 June Seok Heo  ;  Seung Gwan Lee  ;  Hyun Ok Kim 
Citation
 Stem Cells International, Vol.2017 : 6921703, 2017 
Journal Title
 Stem Cells International 
ISSN
 1687-966X 
Issue Date
2017
Abstract
Mesenchymal stem cells (MSCs) are a promising tool for studying intractable diseases. Unfortunately, MSCs can easily undergo cellular senescence during in vitro expansion by losing stemness. The aim of this study was to improve the stemness and differentiation of MSCs by using glabridin, a natural flavonoid. Assessments of cell viability, cell proliferation, β-galactosidase activity, differentiation, and gene expression by reverse transcription PCR were subsequently performed in the absence or presence of glabridin. Glabridin enhanced the self-renewal capacity of MSCs, as indicated by the upregulation of the OCT4 gene. In addition, it resulted in an increase in the osteogenic differentiation potential by inducing the expression of osteogenesis-related genes such as DLX5 and RUNX2. We confirmed that glabridin improved the osteogenesis of MSCs with a significant elevation in the expression of OSTEOCALCIN and OSTEOPONTIN genes. Taken together, these results suggest that glabridin enhances osteogenic differentiation of MSCs with induction of the OCT4 gene; thus, glabridin could be useful for stem cell-based therapies.
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DOI
10.1155/2017/6921703
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
김현옥(Kim, Hyun Ok) ORCID logo https://orcid.org/0000-0002-4964-1963
허준석(Heo, June Seok)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/161384
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