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Mortalin deficiency suppresses fibrosis and induces apoptosis in keloid spheroids

DC FieldValueLanguage
dc.contributor.author이원재-
dc.date.accessioned2018-07-20T08:18:28Z-
dc.date.available2018-07-20T08:18:28Z-
dc.date.issued2017-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/161076-
dc.description.abstractMortalin (Mot) is a mitochondrial chaperone of the heat shock protein 70 family and it's pro-proliferative and anti-apoptosis functions could be associated with keloid pathogenesis, and blocking of mortalin and its interaction with p53 might be a potential novel target for the treatment of keloid. Therefore, we generated mortalin-specific small hairpin (sh) RNAs (dE1-RGD/GFP/shMot) and introduced into keloid spheroids for examination of its apoptotic and anti-fibrotic effect. On keloid tissues, mortalin expression was higher than adjacent normal tissues and it's protein expressions were activated keloid fibroblasts (KFs). After primary keloid spheroid were transduced with dE1-RGD/GFP/shMot for knockdown of mortalin, expression of type I, III collagen, fibronectin, and elastin was significantly reduced and transforming growth factor-β1, epidermal growth factor receptor (EGFR), Extracellular Signal-Regulated Kinases 1 and 2 (Erk 1/2), and Smad 2/3 complex protein expression were decreased. In addition, increased TUNEL activities and cytochrome C were observed. Further, for examine of mortalin and p53 interaction, we performed immunofluorescence analysis. Knockdown of mortalin relocated p53 to the cell nucleus in primary keloid spheroids by dE1-RGD/GFP/shMot transduction. These results support the utility of knockdown of mortalin to induce apoptosis and reduce ECMs expression in keloid spheroid, which may be highly beneficial in treating keloids.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherNature Publishing Group-
dc.relation.isPartOfSCIENTIFIC REPORTS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleMortalin deficiency suppresses fibrosis and induces apoptosis in keloid spheroids-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Plastic Surgery & Reconstructive Surgery-
dc.contributor.googleauthorWon Jai Lee-
dc.contributor.googleauthorHyo Min Ahn-
dc.contributor.googleauthorYoujin Na-
dc.contributor.googleauthorRenu Wadhwa-
dc.contributor.googleauthorJinWoo Hong-
dc.contributor.googleauthorChae-Ok Yun-
dc.identifier.doi10.1038/s41598-017-13485-y-
dc.contributor.localIdA03005-
dc.relation.journalcodeJ02646-
dc.identifier.eissn2045-2322-
dc.identifier.pmid29021584-
dc.contributor.alternativeNameLee, Won Jai-
dc.contributor.affiliatedAuthorLee, Won Jai-
dc.citation.volume7-
dc.citation.number1-
dc.citation.startPage12957-
dc.identifier.bibliographicCitationSCIENTIFIC REPORTS, Vol.7(1) : 12957, 2017-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers

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