Cited 15 times in
Mortalin deficiency suppresses fibrosis and induces apoptosis in keloid spheroids
DC Field | Value | Language |
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dc.contributor.author | 이원재 | - |
dc.date.accessioned | 2018-07-20T08:18:28Z | - |
dc.date.available | 2018-07-20T08:18:28Z | - |
dc.date.issued | 2017 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/161076 | - |
dc.description.abstract | Mortalin (Mot) is a mitochondrial chaperone of the heat shock protein 70 family and it's pro-proliferative and anti-apoptosis functions could be associated with keloid pathogenesis, and blocking of mortalin and its interaction with p53 might be a potential novel target for the treatment of keloid. Therefore, we generated mortalin-specific small hairpin (sh) RNAs (dE1-RGD/GFP/shMot) and introduced into keloid spheroids for examination of its apoptotic and anti-fibrotic effect. On keloid tissues, mortalin expression was higher than adjacent normal tissues and it's protein expressions were activated keloid fibroblasts (KFs). After primary keloid spheroid were transduced with dE1-RGD/GFP/shMot for knockdown of mortalin, expression of type I, III collagen, fibronectin, and elastin was significantly reduced and transforming growth factor-β1, epidermal growth factor receptor (EGFR), Extracellular Signal-Regulated Kinases 1 and 2 (Erk 1/2), and Smad 2/3 complex protein expression were decreased. In addition, increased TUNEL activities and cytochrome C were observed. Further, for examine of mortalin and p53 interaction, we performed immunofluorescence analysis. Knockdown of mortalin relocated p53 to the cell nucleus in primary keloid spheroids by dE1-RGD/GFP/shMot transduction. These results support the utility of knockdown of mortalin to induce apoptosis and reduce ECMs expression in keloid spheroid, which may be highly beneficial in treating keloids. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Mortalin deficiency suppresses fibrosis and induces apoptosis in keloid spheroids | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Plastic Surgery & Reconstructive Surgery | - |
dc.contributor.googleauthor | Won Jai Lee | - |
dc.contributor.googleauthor | Hyo Min Ahn | - |
dc.contributor.googleauthor | Youjin Na | - |
dc.contributor.googleauthor | Renu Wadhwa | - |
dc.contributor.googleauthor | JinWoo Hong | - |
dc.contributor.googleauthor | Chae-Ok Yun | - |
dc.identifier.doi | 10.1038/s41598-017-13485-y | - |
dc.contributor.localId | A03005 | - |
dc.relation.journalcode | J02646 | - |
dc.identifier.eissn | 2045-2322 | - |
dc.identifier.pmid | 29021584 | - |
dc.contributor.alternativeName | Lee, Won Jai | - |
dc.contributor.affiliatedAuthor | Lee, Won Jai | - |
dc.citation.volume | 7 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 12957 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, Vol.7(1) : 12957, 2017 | - |
dc.identifier.rimsid | 60966 | - |
dc.type.rims | ART | - |
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