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Proteomic Analysis of Serum Amyloid A as a Potential Marker in Intestinal Behçet's Disease

DC FieldValueLanguage
dc.contributor.author김원호-
dc.contributor.author김태일-
dc.contributor.author박수정-
dc.contributor.author이현정-
dc.contributor.author이혜원-
dc.contributor.author천재희-
dc.contributor.author홍성필-
dc.contributor.author김승원-
dc.date.accessioned2018-07-20T08:13:41Z-
dc.date.available2018-07-20T08:13:41Z-
dc.date.issued2017-
dc.identifier.issn0163-2116-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/160989-
dc.description.abstractBACKGROUND/AIMS: Data regarding biomarkers to understand disease pathogenesis and to assess disease activity of intestinal Behçet's disease (BD) are limited. Therefore, we aimed to investigate the differentially expressed proteins in sera from patients with intestinal BD and to search for biomarkers using mass spectrometry-based proteomic analysis. METHODS: Serum samples were pooled for the screening study, and two-dimensional electrophoresis (2-DE) was performed to characterize the proteins present in intestinal BD patients. Candidate protein spots were identified using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS) and bioinformatic analysis. To validate the proteomic results, serum samples from an independent cohort were assessed by enzyme-linked immunosorbent assay. RESULTS: Pooled serum samples were used for 2-DE, and approximately 400 protein spots were detected in the sera of intestinal BD patients. Of the 22 differentially expressed proteins, 3 were successfully identified using MALDI-TOF/TOF MS. The three up-regulated proteins identified in the intestinal BD group included fibrin, apolipoprotein A-IV, and serum amyloid A (SAA). Serum SAA in intestinal BD patients (2.76 ± 2.50 ng/ml) was significantly higher than that in controls (1.68 ± 0.90 ng/ml, p = 0.007), which is consistent with the proteomic results. In addition, the level of IL-1β in patients with intestinal BD (8.96 ± 1.23 pg/ml) was higher than that in controls (5.40 ± 0.15 pg/ml, p = 0.009). SAA released by HT-29 cells was markedly increased by tumor necrosis factor-α (TNF-α) and lipopolysaccharides stimulation. CONCLUSIONS: Our proteomic analysis revealed that SAA was up-regulated in intestinal BD patients.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherSpringer Science + Business Media-
dc.relation.isPartOfDigestive Diseases and Sciences-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHApolipoproteins A/blood-
dc.subject.MESHBehcet Syndrome/blood*-
dc.subject.MESHBiomarkers/blood-
dc.subject.MESHCase-Control Studies-
dc.subject.MESHEnzyme-Linked Immunosorbent Assay-
dc.subject.MESHFemale-
dc.subject.MESHFibrin/analysis-
dc.subject.MESHHT29 Cells-
dc.subject.MESHHumans-
dc.subject.MESHInterleukin-1beta/blood-
dc.subject.MESHIntestinal Diseases/blood*-
dc.subject.MESHMale-
dc.subject.MESHMass Spectrometry-
dc.subject.MESHMiddle Aged-
dc.subject.MESHProteomics/methods*-
dc.subject.MESHSerum Amyloid A Protein/analysis*-
dc.titleProteomic Analysis of Serum Amyloid A as a Potential Marker in Intestinal Behçet's Disease-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorHyun Jung Lee-
dc.contributor.googleauthorJae Hyun Kim-
dc.contributor.googleauthorSeung Won Kim-
dc.contributor.googleauthorHyun Ah Joo-
dc.contributor.googleauthorHye Won Lee-
dc.contributor.googleauthorYou Sun Kim-
dc.contributor.googleauthorSoo Jung Park-
dc.contributor.googleauthorSung Pil Hong-
dc.contributor.googleauthorTae Il Kim-
dc.contributor.googleauthorWon Ho Kim-
dc.contributor.googleauthorYoung-Ho Kim-
dc.contributor.googleauthorJae Hee Cheon-
dc.identifier.doi10.1007/s10620-017-4606-y-
dc.contributor.localIdA00774-
dc.contributor.localIdA01079-
dc.contributor.localIdA01539-
dc.contributor.localIdA03295-
dc.contributor.localIdA03318-
dc.contributor.localIdA04030-
dc.contributor.localIdA04404-
dc.relation.journalcodeJ00737-
dc.identifier.eissn1573-2568-
dc.identifier.pmid28523576-
dc.identifier.urlhttps://link.springer.com/article/10.1007%2Fs10620-017-4606-y-
dc.subject.keywordBiomarkers-
dc.subject.keywordIntestinal Behçet’s disease-
dc.subject.keywordProteomics-
dc.subject.keywordSerum amyloid A-
dc.contributor.alternativeNameKim, Won Ho-
dc.contributor.alternativeNameKim, Tae Il-
dc.contributor.alternativeNamePark, Soo Jung-
dc.contributor.alternativeNameLee, Hyun Jung-
dc.contributor.alternativeNameLee, Hye Won-
dc.contributor.alternativeNameCheon, Jae Hee-
dc.contributor.alternativeNameHong, Sung Pil-
dc.contributor.affiliatedAuthorKim, Won Ho-
dc.contributor.affiliatedAuthorKim, Tae Il-
dc.contributor.affiliatedAuthorPark, Soo Jung-
dc.contributor.affiliatedAuthorLee, Hyun Jung-
dc.contributor.affiliatedAuthorLee, Hye Won-
dc.contributor.affiliatedAuthorCheon, Jae Hee-
dc.contributor.affiliatedAuthorHong, Sung Pil-
dc.citation.volume62-
dc.citation.number8-
dc.citation.startPage1953-
dc.citation.endPage1962-
dc.identifier.bibliographicCitationDigestive Diseases and Sciences, Vol.62(8) : 1953-1962, 2017-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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