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Tunable Surface Repellency Maintains Stemness and Redox Capacity of Human Mesenchymal Stem Cells

Authors
 Daniel A. Balikov  ;  Spencer W. Crowder  ;  Timothy C. Boire  ;  Jung Bok Lee  ;  Mukesh K. Gupta  ;  Aidan M. Fenix  ;  Holley N. Lewis  ;  Caitlyn M. Ambrose  ;  Philip A. Short  ;  Chang Soo Kim  ;  Dylan T. Burnette  ;  Matthew A. Reilly  ;  N. Sanjeeva Murthy  ;  Mi-Lan Kang  ;  Won Shik Kim  ;  Hak-Joon Sung 
Citation
 ACS APPLIED MATERIALS & INTERFACES, Vol.9(27) : 22994-23006, 2017 
Journal Title
 ACS APPLIED MATERIALS & INTERFACES 
ISSN
 1944-8244 
Issue Date
2017
Keywords
biomaterials ; cell-matrix interface ; polymers ; regenerative medicine ; stem cells
Abstract
Human bone marrow derived mesenchymal stem cells (hMSCs) hold great promise for regenerative medicine due to their multipotent differentiation capacity and immunomodulatory capabilities. Substantial research has elucidated mechanisms by which extracellular cues regulate hMSC fate decisions, but considerably less work has addressed how material properties can be leveraged to maintain undifferentiated stem cells. Here, we show that synthetic culture substrates designed to exhibit moderate cell-repellency promote high stemness and low oxidative stress-two indicators of naïve, healthy stem cells-in commercial and patient-derived hMSCs. Furthermore, the material-mediated effect on cell behavior can be tuned by altering the molar percentage (mol %) and/or chain length of poly(ethylene glycol) (PEG), the repellant block linked to hydrophobic poly(ε-caprolactone) (PCL) in the copolymer backbone. Nano- and angstrom-scale characterization of the cell-material interface reveals that PEG interrupts the adhesive PCL domains in a chain-length-dependent manner; this prevents hMSCs from forming mature focal adhesions and subsequently promotes cell-cell adhesions that require connexin-43. This study is the first to demonstrate that intrinsic properties of synthetic materials can be tuned to regulate the stemness and redox capacity of hMSCs and provides new insight for designing highly scalable, programmable culture platforms for clinical translation.
Files in This Item:
T201703178.pdf Download
DOI
10.1021/acsami.7b06103
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Kim, Won Shik(김원식)
Sung, Hak-Joon(성학준) ORCID logo https://orcid.org/0000-0003-2312-2484
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160753
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