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B-cell translocation gene 1 is downregulated by promoter methylation in ovarian carcinoma

Authors
 Ji-Ye Kim  ;  Sung-Im Do  ;  Go Eun Bae  ;  Hyun-Soo Kim 
Citation
 JOURNAL OF CANCER, Vol.8(14) : 2669-2675, 2017 
Journal Title
 JOURNAL OF CANCER 
Issue Date
2017
Keywords
B-cell translocation gene 1 ; downregulation ; ovarian carcinoma ; ovary ; promoter hypermethylation
Abstract
A better understanding of tumor biology is important in the identification of molecules that are downregulated in malignancy and in determining their role in tumor suppression. B-cell translocation gene 1 (BTG1) has been shown to act as a tumor suppressor in several types of human malignancy. In this study, we analyzed BTG1 expression in ovarian carcinoma cell lines, and we investigated the mechanism underlying the observed alterations. The methylation status of the BTG1 promoter region was determined by methylation-specific polymerase chain reaction, and the effect of demethylation on BTG1 expression was analyzed. BTG1 protein expression in ovarian high-grade serous carcinoma tissue samples was evaluated using immunohistochemistry. BTG1 mRNA and protein expression were reduced in ovarian carcinoma cells. In BTG1-silenced ovarian cancer cells, the BTG1 promoter was highly methylated. Treatment with 5-aza-deoxycytidine significantly elevated BTG1 mRNA and protein expression. Immunostaining demonstrated that BTG1 expression was significantly lower in ovarian carcinoma tissue samples than nonpathological ovaries and fallopian tubes. We demonstrated that BTG1 silencing in ovarian carcinoma occurs through epigenetic repression and is involved in the ovarian carcinogenesis. Our data suggest that BTG1 is a potential therapeutic target for patients with ovarian carcinoma.
Files in This Item:
T201703138.pdf Download
DOI
10.7150/jca.21037
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun-Soo(김현수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160734
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