0 46

Cited 6 times in

Differences of lung microbiome in patients with clinically stable and exacerbated bronchiectasis.

Authors
 Min Kwang Byun  ;  Joon Chang  ;  Hyung Jung Kim  ;  Seok Hoon Jeong 
Citation
 PLoS One, Vol.12(8) : e0183553, 2017 
Journal Title
 PLoS One 
Issue Date
2017
MeSH
Adult ; Aged ; Bacteria/classification ; Bacteria/genetics ; Bronchiectasis/microbiology ; Bronchiectasis/pathology ; Bronchoalveolar Lavage Fluid ; Female ; Humans ; Lung/microbiology ; Male ; Microbiota ; Middle Aged ; Polymerase Chain Reaction ; RNA, Ribosomal, 16S/genetics
Abstract
BACKGROUND: Molecular-based diagnostic techniques can compensate for the inherent limitations of culture-based microbiology and provide a more comprehensive description of an entire community of bacteria at a particular anatomical site. Using culture-independent DNA-based molecular techniques, the aim of the present study was to characterize, differentiate, and compare the composition of lower airway bacterial microbiome between clinically stable and acutely infected patients with bronchiectasis experiencing exacerbation. METHODS: Patients with clinically stable bronchiectasis and those experiencing acutely exacerbated bronchiectasis were recruited. All patients underwent bronchoscopy. Paired sputum and bronchoalveolar lavage (BAL) samples were collected for microbiological tests. Molecular analysis was performed for BAL samples using 16S ribosomal RNA (rRNA) gene sequencing. RESULTS: The mean age of the 14 recruited patients was 60 years (range 42 to 78 years), and nine (64%) were female. Using quantitative culture and 16S rRNA sequencing, the common organisms identified from 14 BAL samples were Haemophilus influenzae, Pseudomonas aeruginosa and Moraxella catarrhalis, and Prevotella. Molecular techniques revealed Prevotella and Veillonella as potentially pathogenic anaerobic species. 16S rRNA gene sequencing yielded similar relative abundances and distributions of taxa in the stable and exacerbated bronchiectasis groups. Alpha diversity with richness, Simpson's and Shannon indices, and beta diversity using principal coordinate analysis revealed no significant differences in lung microbiome between patients with clinically stable and exacerbated bronchiectasis. CONCLUSION: Culture-based microbiological and molecular-based techniques did not reveal significant differences in the lung microbiome of patients who were clinically stable and those experiencing exacerbated bronchiectasis. Patient-specific microbial communities were dominated by one or several genera, regardless of clinical status. DNA sequencing could identify potentially pathogenic organisms unable to be identified using microbiological methods.
DOI
10.1371/journal.pone.0183553
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
김형중(Kim, Hyung Jung) ORCID logo https://orcid.org/0000-0003-2498-0683
변민광(Byun, Min Kwang) ORCID logo https://orcid.org/0000-0003-1525-1745
장준(Chang, Joon) ORCID logo https://orcid.org/0000-0003-4542-6841
정석훈(Jeong, Seok Hoon) ORCID logo https://orcid.org/0000-0001-9290-897X
Export
RIS (EndNote)
XLS (Excel)
XML
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160692
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse