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Cross‑reactivity between group-5 and -21 mite allergens from Dermatophagoides farinae, Tyrophagus putrescentiae and Blomia tropicalis

DC Field Value Language
dc.contributor.author김형표-
dc.contributor.author용태순-
dc.contributor.author이명희-
dc.contributor.author정경영-
dc.contributor.author정경용-
dc.date.accessioned2018-03-26T17:06:35Z-
dc.date.available2018-03-26T17:06:35Z-
dc.date.issued2015-
dc.identifier.issn1791-2997-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/157199-
dc.description.abstractGroup-5 and group-21 allergens, produced by house dust mites and storage mites are 36.6-55.8% identical in their sequences and are recognized by at least 50% of immunoglobulin (Ig)E from the sera of individuals allergic to dust mites. In the present study, recombinant group-5 and ‑21 allergens from three mite species, Dermatophagoides farinae (rDer f 5 and 21), Tyrophagus putrescentiae (rTyr p 5 and 21), and Blomia tropicalis (rBlo t 5 and 21), were purified from Escherichia coli, and the IgE reactivities and cross‑reactivities of these allergen variants were assessed. The IgE binding frequencies of rDer f 5, rDer f 21, rTyr p 5, rTyr p 21, rBlo t and rBlo t 21 proteins were 64.95, 65.98, 30.41, 41.24, 30.93 and 21.65%, respectively. The IgE reactivity of rDer f 5 correlated highly with that of rDer f 21 (r=0.733). rTyr p 5 exhibited the highest level of correlation with rTyr p 21 (r=0.950), while the correlation of rBlo t 5 with rBlo t 21 was the lowest observed (r=0.104). The binding of IgE to rDer f 5 and rDer f 21 was not inhibited by any allergens but themselves. While rDer f 5 inhibited only 60.3% of IgE binding to rBlo t 5, rDer f 21 exhibited a high inhibitory effect against rTyr p 5 (93.01%), rTyr p 21 (92.12%), rBlo t 5 (86.97%) and rBlo t 21 (70.30%), implying cross‑reactivity among mite species. The results of the present study demonstrated that the majority of the IgE reactivity to group-5 and -21 storage mite allergens is due to cross‑reaction. It is therefore imperative to develop an accurate, component‑resolved diagnosis for dust mite allergies.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherD. A. Spandidos-
dc.relation.isPartOfMOLECULAR MEDICINE REPORTS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdolescent-
dc.subject.MESHAdult-
dc.subject.MESHAllergens/chemistry-
dc.subject.MESHAllergens/genetics-
dc.subject.MESHAllergens/immunology*-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHAnimals-
dc.subject.MESHAntibody Specificity/immunology-
dc.subject.MESHAntigens, Dermatophagoides/chemistry-
dc.subject.MESHAntigens, Dermatophagoides/genetics-
dc.subject.MESHAntigens, Dermatophagoides/immunology*-
dc.subject.MESHAsthma/blood-
dc.subject.MESHAsthma/immunology-
dc.subject.MESHChild-
dc.subject.MESHCross Reactions/immunology*-
dc.subject.MESHDermatophagoides farinae/genetics-
dc.subject.MESHDermatophagoides farinae/immunology*-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHHypersensitivity/blood-
dc.subject.MESHHypersensitivity/immunology-
dc.subject.MESHImmunoglobulin E/blood-
dc.subject.MESHImmunoglobulin E/immunology-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHPyroglyphidae/genetics-
dc.subject.MESHPyroglyphidae/immunology*-
dc.subject.MESHRecombinant Proteins/genetics-
dc.subject.MESHRecombinant Proteins/immunology-
dc.subject.MESHSequence Alignment-
dc.subject.MESHYoung Adult-
dc.titleCross‑reactivity between group-5 and -21 mite allergens from Dermatophagoides farinae, Tyrophagus putrescentiae and Blomia tropicalis-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Environmental Medical Biology-
dc.contributor.googleauthorCHUNG‑RYUL KIM-
dc.contributor.googleauthorKYOUNG YONG JEONG-
dc.contributor.googleauthorMYUNG‑HEE YI-
dc.contributor.googleauthorHYOUNG‑PYO KIM-
dc.contributor.googleauthorHO‑JOON SHIN-
dc.contributor.googleauthorTAI‑SOON YONG-
dc.identifier.doi10.3892/mmr.2015.4093-
dc.contributor.localIdA01163-
dc.contributor.localIdA02424-
dc.contributor.localIdA02761-
dc.contributor.localIdA03572-
dc.relation.journalcodeJ02261-
dc.identifier.eissn1791-3004-
dc.identifier.pmid26238285-
dc.identifier.urlhttps://www.spandidos-publications.com/mmr/12/4/5467-
dc.contributor.alternativeNameKim, Hyoung Pyo-
dc.contributor.alternativeNameYong, Tai Soon-
dc.contributor.alternativeNameYi, Myung Hee-
dc.contributor.alternativeNameChung, Kyung Young-
dc.contributor.affiliatedAuthorKim, Hyoung Pyo-
dc.contributor.affiliatedAuthorYong, Tai Soon-
dc.contributor.affiliatedAuthorYi, Myung Hee-
dc.contributor.affiliatedAuthorJeong, Kyoung Yong-
dc.citation.volume12-
dc.citation.number4-
dc.citation.startPage5467-
dc.citation.endPage5474-
dc.identifier.bibliographicCitationMOLECULAR MEDICINE REPORTS, Vol.12(4) : 5467-5474, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers

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