103 135

Cited 2 times in

Genetic Diversity of Schistosoma haematobium Eggs Isolated from Human Urine in Sudan

Authors
 Juan-Hua Quan  ;  In-Wook Choi  ;  Hassan Ahmed Hassan Ahmed Ismail  ;  Abdoelohab Saed Mohamed  ;  Hoo-Gn Jeong  ;  Jin-Su Lee  ;  Sung-Tae Hong  ;  Tai-Soon Yong  ;  Guang-Ho Cha  ;  Young-Ha Lee 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.53(3) : 271-277, 2015 
Journal Title
 KOREAN JOURNAL OF PARASITOLOGY 
ISSN
 0023-4001 
Issue Date
2015
MeSH
Adolescent ; Animals ; Base Sequence ; Child ; DNA, Helminth/genetics ; Female ; Genetic Variation* ; Genotype ; Humans ; Male ; Molecular Sequence Data ; Ovum/classification ; Ovum/cytology ; Parasite Egg Count ; Polymorphism, Restriction Fragment Length ; Schistosoma haematobium/genetics* ; Schistosoma haematobium/isolation & purification* ; Schistosoma haematobium/physiology ; Schistosomiasis haematobia/diagnosis ; Schistosomiasis haematobia/epidemiology ; Schistosomiasis haematobia/parasitology* ; Schistosomiasis haematobia/urine ; Students ; Sudan/epidemiology ; Urine/parasitology*
Keywords
ITS2 ; PCR-restriction fragment length polymorphism (PCR-RFLP) ; Schistosoma haematobium ; Sudan
Abstract
The genetic diversity of Schistosoma haematobium remains largely unstudied in comparison to that of Schistosoma mansoni. To characterize the extent of genetic diversity in S. haematobium among its definitive host (humans), we collected S. haematobium eggs from the urine of 73 infected schoolchildren at 5 primary schools in White Nile State, Sudan, and then performed a randomly amplified polymorphic DNA marker ITS2 by PCR-RFLP analysis. Among 73 S. haematobium egg-positive cases, 13 were selected based on the presence of the S. haematobium satellite markers A4 and B2 in their genomic DNA, and used for RFLP analysis. The 13 samples were subjected to an RFLP analysis of the S. haematobium ITS2 region; however, there was no variation in size among the fragments. Compared to the ITS2 sequences obtained for S. haematobium from Kenya, the nucleotide sequences of the ITS2 regions of S. haematobium from 4 areas in Sudan were consistent with those from Kenya (> 99%). In this study, we demonstrate for the first time that most of the S. haematobium population in Sudan consists of a pan-African S. haematobium genotype; however, we also report the discovery of Kenyan strain inflow into White Nile, Sudan.
Files in This Item:
T201505958.pdf Download
DOI
10.3347/kjp.2015.53.3.271
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/157196
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links