Cited 14 times in
Selective serotonin reuptake inhibitors facilitate ANO6 (TMEM16F) current activation and phosphatidylserine exposure
DC Field | Value | Language |
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dc.contributor.author | 송재우 | - |
dc.contributor.author | 이민구 | - |
dc.contributor.author | 전익현 | - |
dc.contributor.author | 정진세 | - |
dc.date.accessioned | 2018-03-26T17:02:25Z | - |
dc.date.available | 2018-03-26T17:02:25Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0031-6768 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/157102 | - |
dc.description.abstract | Anoctamin 6 (ANO6) is a member of the recently identified TMEM16/anoctamin protein family comprising Ca(2+)-activated Cl(-) channels that generate outward-rectifying ionic currents in response to intracellular Ca(2+) increase. ANO6 is also essential for Ca(2+)-dependent phospholipid scrambling required for blood coagulation. Selective serotonin reuptake inhibitors (SSRIs)--fluoxetine, sertraline, and paroxetine-that are used for the treatment of major depressive disorders can increase the risk of upper gastrointestinal bleeding after chronic treatment. However, at the earlier stage of intake, which is 1-7 days after the treatment, the possibility of blood coagulation might also increase, but transiently. Therefore, in this study, we investigated whether therapeutic SSRI concentrations affected the Cl(-) current or phospholipid scrambling activity of ANO6 by assessing ANO6 currents (I ANO6), phosphatidylserine (PS) exposure, and platelet aggregation. In the whole-cell patch mode, SSRIs facilitated Ca(2+)-dependent activation of IANO6 in ANO6-transfected cells, as evidenced by a significant decrease in the delay of IANO6 generation. On the other hand, in the inside-out patch clamp configuration, SSRIs showed an inhibitory effect on ANO6 currents, suggesting that SSRIs activate ANO6 via an indirect mechanism in intact cells. SSRIs also facilitated Ca(2+)-dependent PS exposure and α-thrombin-induced platelet aggregation. These results indicate that SSRIs at clinically relevant concentrations promote Ca(2+)-dependent activation of ANO6, which may have potential clinical implications such as the underlying mechanism of SSRI-induced adverse drug reactions. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | German, English | - |
dc.publisher | Springer | - |
dc.relation.isPartOf | PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Anoctamins | - |
dc.subject.MESH | Blood Coagulation/drug effects | - |
dc.subject.MESH | Calcium Signaling/drug effects | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Chloride Channels/metabolism | - |
dc.subject.MESH | HEK293 Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Patch-Clamp Techniques | - |
dc.subject.MESH | Phosphatidylserines/metabolism* | - |
dc.subject.MESH | Phospholipid Transfer | - |
dc.subject.MESH | Proteins/drug effects | - |
dc.subject.MESH | Phospholipid Transfer Proteins/metabolism* | - |
dc.subject.MESH | Plasmids/genetics | - |
dc.subject.MESH | Platelet Aggregation/drug effects | - |
dc.subject.MESH | RNA, Small Interfering/pharmacology | - |
dc.subject.MESH | Serotonin Uptake Inhibitors/pharmacology* | - |
dc.subject.MESH | Thrombin/pharmacology | - |
dc.subject.MESH | Transfection | - |
dc.title | Selective serotonin reuptake inhibitors facilitate ANO6 (TMEM16F) current activation and phosphatidylserine exposure | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Laboratory Medicine | - |
dc.contributor.googleauthor | Hyun Jong Kim | - |
dc.contributor.googleauthor | Ikhyun Jun | - |
dc.contributor.googleauthor | Jae Seok Yoon | - |
dc.contributor.googleauthor | Jinsei Jung | - |
dc.contributor.googleauthor | Yung Kyu Kim | - |
dc.contributor.googleauthor | Woo Kyung Kim | - |
dc.contributor.googleauthor | Byung Joo Kim | - |
dc.contributor.googleauthor | Jaewoo Song | - |
dc.contributor.googleauthor | Sung Joon Kim | - |
dc.contributor.googleauthor | Joo Hyun Nam | - |
dc.contributor.googleauthor | Min Goo Lee | - |
dc.identifier.doi | 10.1007/s00424-015-1692-6 | - |
dc.contributor.localId | A03742 | - |
dc.contributor.localId | A02054 | - |
dc.contributor.localId | A02781 | - |
dc.contributor.localId | A03541 | - |
dc.relation.journalcode | J02502 | - |
dc.identifier.eissn | 1432-2013 | - |
dc.identifier.pmid | 25630304 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs00424-015-1692-6 | - |
dc.subject.keyword | Anoctamin 6 | - |
dc.subject.keyword | Calcium-activated Cl− channels | - |
dc.subject.keyword | Selective serotonin reuptake inhibitors | - |
dc.subject.keyword | TMEM16F | - |
dc.contributor.alternativeName | Song, Jae Woo | - |
dc.contributor.alternativeName | Lee, Min Goo | - |
dc.contributor.alternativeName | Jun, Ik Hyun | - |
dc.contributor.alternativeName | Jung, Jinsei | - |
dc.contributor.affiliatedAuthor | Jung, Jinsei | - |
dc.contributor.affiliatedAuthor | Song, Jae Woo | - |
dc.contributor.affiliatedAuthor | Lee, Min Goo | - |
dc.contributor.affiliatedAuthor | Jun, Ik Hyun | - |
dc.citation.volume | 467 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 2243 | - |
dc.citation.endPage | 2256 | - |
dc.identifier.bibliographicCitation | PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, Vol.467(11) : 2243-2256, 2015 | - |
dc.identifier.rimsid | 41665 | - |
dc.type.rims | ART | - |
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