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The Role of Foxo3 in Leydig Cells.

DC Field Value Language
dc.contributor.author이은직-
dc.contributor.author최영숙-
dc.date.accessioned2018-03-26T16:53:53Z-
dc.date.available2018-03-26T16:53:53Z-
dc.date.issued2015-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/156935-
dc.description.abstractPURPOSE: Foxo3 in female reproduction has been reported to regulate proliferation of granulose cells that form follicles. There are no reports so far that discuss on the role of Foxo3 in males. This study was designed to outline the role of Foxo3 in the testes. MATERIALS AND METHODS: Testes from mice at birth to postpartum week (PPW) 5 were isolated and examined for the expression of Foxo3 using immunostaining. To elucidate role of Foxo3 in Leydig cells, R2C cells were treated with luteinizing hormone (LH) and the phosphorylation of Foxo3. Testosterone and steroidogenic acute regulatory (StAR) protein levels were measured after constitutive active [triple mutant (TM)] human FOXO3 adenovirus was transduced and StAR promoter assay was performed. RESULTS: Foxo3 expression in the testicles started from birth and lasted until PPW 3. After PPW 3, most Foxo3 expression occurred in the nuclei of Leydig cells; however, at PPW 5, Foxo3 was expressed in both the nucleus and cytoplasm. When R2C cells were treated with luteinizing hormone, Foxo3 phosphorylation levels by AKT increased. After blocking the PI3K pathway, LH-induced phosphorylated Foxo3 levels decreased, indicating that LH signaling regulates Foxo3 localization. When active FOXO3-TM adenovirus was introduced into a Leydig tumor cell line, the concentrations of testosterone and StAR protein decreased. When FOXO3 and a StAR promoter vector were co-transfected into HEK293 cells for a reporter assay, FOXO3 inhibited the StAR promoter. CONCLUSION: FOXO3 affects testosterone synthesis by inhibiting the formation of StAR protein. LH hormone, meanwhile, influences Foxo3 localization, mediating its function.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherYonsei University-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCell Nucleus/metabolism-
dc.subject.MESHCellular Senescence/physiology*-
dc.subject.MESHCytoplasm/metabolism-
dc.subject.MESHForkhead Box Protein O3-
dc.subject.MESHForkhead Transcription Factors/metabolism*-
dc.subject.MESHHEK293 Cells-
dc.subject.MESHHumans-
dc.subject.MESHLeydig Cells/drug effects*-
dc.subject.MESHLeydig Cells/enzymology*-
dc.subject.MESHLeydig Cells/metabolism-
dc.subject.MESHLuteinizing Hormone/blood-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHPhosphatidylinositol 3-Kinases-
dc.subject.MESHPhosphoproteins/metabolism-
dc.subject.MESHPhosphorylation-
dc.subject.MESHSignal Transduction/drug effects-
dc.subject.MESHTestosterone/blood-
dc.subject.MESHTestosterone/metabolism*-
dc.titleThe Role of Foxo3 in Leydig Cells.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorYoung Suk Choi-
dc.contributor.googleauthorJoo Eun Song-
dc.contributor.googleauthorByung Soo Kong-
dc.contributor.googleauthorJae Won Hong-
dc.contributor.googleauthorSilvia Novelli-
dc.contributor.googleauthorEun Jig Lee-
dc.identifier.doi10.3349/ymj.2015.56.6.1590-
dc.contributor.localIdA03050-
dc.contributor.localIdA04693-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid26446641-
dc.subject.keywordFoxo3-
dc.subject.keywordLeydig cell-
dc.subject.keywordStAR-
dc.subject.keywordtestosterone-
dc.contributor.alternativeNameLee, Eun Jig-
dc.contributor.alternativeNameChoi, Young Suk-
dc.contributor.affiliatedAuthorLee, Eun Jig-
dc.contributor.affiliatedAuthorChoi, Young Suk-
dc.citation.volume56-
dc.citation.number6-
dc.citation.startPage1590-
dc.citation.endPage1596-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.56(6) : 1590-1596, 2015-
dc.identifier.rimsid41244-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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