Cited 9 times in
Double transduction of a Cre/LoxP lentiviral vector: a simple method to generate kidney cell-specific knockdown mice
DC Field | Value | Language |
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dc.contributor.author | 강신욱 | - |
dc.contributor.author | 강혜영 | - |
dc.contributor.author | 김성훈 | - |
dc.contributor.author | 남보영 | - |
dc.contributor.author | 박정탁 | - |
dc.contributor.author | 박지민 | - |
dc.contributor.author | 엄재은 | - |
dc.contributor.author | 오형중 | - |
dc.contributor.author | 유태현 | - |
dc.contributor.author | 팽지선 | - |
dc.contributor.author | 한승혁 | - |
dc.date.accessioned | 2018-03-26T16:53:19Z | - |
dc.date.available | 2018-03-26T16:53:19Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1931-857X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/156922 | - |
dc.description.abstract | In a lentivirus-based gene delivery system, the incorporated gene is continuously expressed for a long time. In this study, we devised a simple way to knock down a specific gene in a kidney cell-specific pattern in adult mice by lentivirus-assisted transfer of short hairpin RNA (shRNA). Kidney collecting duct (CD)-specific aquaporin-3 (AQP3)-knockdown mice were generated by consecutive injection of Hoxb7-Cre-expressing lentivirus (LV-Hoxb7 Cre) and loxP-AQP3 shRNA-expressing lentivirus (LV-loxP shAQP3) in adult C57BL6/J mice. LV-Hoxb7 Cre was designed to express mCherry, while LV-loxP shAQP3 was designed with a floxed enhanced green fluorescent protein (EGFP)-tagged stop sequence, and thus EGFP would be expressed only in the absence of Cre recombination. In mice treated with LV-Hoxb7 Cre alone, mCherry protein expression, which indicates the presence of Cre recombinase, occurred only in CD cells. However, LV-loxP shAQP3 injection alone resulted in an increase in EGFP expression in all kidney cells, indicating the transcription of the floxed region. When LV-Hoxb7 Cre and LV-loxP shAQP3 were sequentially transduced, EGFP expression was attenuated while mCherry expression was sustained in CD cells, demonstrating a CD cell-specific recombination of the floxed region. AQP3 expression in mice injected with LV-Hoxb7 Cre or LV-loxP shAQP3 alone did not differ, but consecutive injection of LV-Hoxb7 Cre and LV-loxP shAQP3 significantly reduced AQP3 expression in CD cells. However, the expression levels of AQP3 were not altered in other cell types. Double transduction of Cre- and loxP-based lentivirus can easily generate kidney cell-specific knockdown mice, and this method might be applicable to other species. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | American Physiological Society | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Gene Knockdown Techniques | - |
dc.subject.MESH | Gene Transfer Techniques | - |
dc.subject.MESH | Genetic Vectors* | - |
dc.subject.MESH | Green Fluorescent Proteins/genetics | - |
dc.subject.MESH | Integrases/genetics* | - |
dc.subject.MESH | Kidney/cytology* | - |
dc.subject.MESH | Lentivirus | - |
dc.subject.MESH | Mice, Transgenic | - |
dc.subject.MESH | Promoter Regions, Genetic/genetics | - |
dc.title | Double transduction of a Cre/LoxP lentiviral vector: a simple method to generate kidney cell-specific knockdown mice | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Internal Medicine | - |
dc.contributor.googleauthor | Bo Young Nam | - |
dc.contributor.googleauthor | Dong Ki Kim | - |
dc.contributor.googleauthor | Jung Tak Park | - |
dc.contributor.googleauthor | Hye-Young Kang | - |
dc.contributor.googleauthor | Jisun Paeng | - |
dc.contributor.googleauthor | Seonghun Kim | - |
dc.contributor.googleauthor | Jimin Park | - |
dc.contributor.googleauthor | Jae Eun Um | - |
dc.contributor.googleauthor | Hyung Jung Oh | - |
dc.contributor.googleauthor | Seung Hyeok Han | - |
dc.contributor.googleauthor | Tae-Hyun Yoo | - |
dc.contributor.googleauthor | Shin-Wook Kang | - |
dc.identifier.doi | 10.1152/ajprenal.00251.2015 | - |
dc.contributor.localId | A00053 | - |
dc.contributor.localId | A00096 | - |
dc.contributor.localId | A00598 | - |
dc.contributor.localId | A01251 | - |
dc.contributor.localId | A01654 | - |
dc.contributor.localId | A05243 | - |
dc.contributor.localId | A05250 | - |
dc.contributor.localId | A02417 | - |
dc.contributor.localId | A02526 | - |
dc.contributor.localId | A04241 | - |
dc.contributor.localId | A04304 | - |
dc.relation.journalcode | J00108 | - |
dc.identifier.eissn | 1522-1466 | - |
dc.identifier.pmid | 26377795 | - |
dc.identifier.url | http://www.physiology.org/doi/abstract/10.1152/ajprenal.00251.2015 | - |
dc.subject.keyword | Cre/LoxP | - |
dc.subject.keyword | cell-specific knockdown | - |
dc.subject.keyword | lentiviral vector | - |
dc.contributor.alternativeName | Kang, Shin Wook | - |
dc.contributor.alternativeName | Kang, Hye Young | - |
dc.contributor.alternativeName | Kim, Seonghun | - |
dc.contributor.alternativeName | Nam, Bo Young | - |
dc.contributor.alternativeName | Park, Jung Tak | - |
dc.contributor.alternativeName | Park, Jimin | - |
dc.contributor.alternativeName | Um, Jae Eun | - |
dc.contributor.alternativeName | Oh, Hyung Jung | - |
dc.contributor.alternativeName | Yoo, Tae Hyun | - |
dc.contributor.alternativeName | Paeng, Ji Sun | - |
dc.contributor.alternativeName | Han, Seung Hyeok | - |
dc.contributor.affiliatedAuthor | Kang, Shin Wook | - |
dc.contributor.affiliatedAuthor | Kang, Hye Young | - |
dc.contributor.affiliatedAuthor | Kim, Seonghun | - |
dc.contributor.affiliatedAuthor | Nam, Bo Young | - |
dc.contributor.affiliatedAuthor | Park, Jung Tak | - |
dc.contributor.affiliatedAuthor | Park, Jimin | - |
dc.contributor.affiliatedAuthor | Um, Jae Eun | - |
dc.contributor.affiliatedAuthor | Oh, Hyung Jung | - |
dc.contributor.affiliatedAuthor | Yoo, Tae Hyun | - |
dc.contributor.affiliatedAuthor | Paeng, Ji Sun | - |
dc.contributor.affiliatedAuthor | Han, Seung Hyeok | - |
dc.citation.volume | 309 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | F1060 | - |
dc.citation.endPage | F1069 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, Vol.309(12) : F1060-F1069, 2015 | - |
dc.identifier.rimsid | 41231 | - |
dc.type.rims | ART | - |
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