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Autophagy contributes to the chemo-resistance of non-small cell lung cancer in hypoxic conditions

DC FieldValueLanguage
dc.contributor.author김대준-
dc.contributor.author김영삼-
dc.contributor.author신주혜-
dc.contributor.author심효섭-
dc.contributor.author이진구-
dc.contributor.author이창영-
dc.contributor.author정경영-
dc.date.accessioned2018-03-26T16:43:31Z-
dc.date.available2018-03-26T16:43:31Z-
dc.date.issued2015-
dc.identifier.issn1465-9921-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/156755-
dc.description.abstractBACKGROUND: The development of chemo-resistance in non-small lung cancer is a major obstacle in treating patients. Hypoxia is a commonly faced microenvironment in solid tumor and suggested to be related to both autophagy and chemo-resistance. METHODS: In this study, we investigated the role of hypoxia-induced autophagy in acquiring chemo-resistance in both cancer cell (A549) and human cancer tissue RESULTS: Hypoxic exposure (1 % O2) of A549 cell stimulated autophagic induction in cancer cells, shown by increase of LC3BI to LC3BII conversion and decrease of p62/sequestosome1 in Western blot, increased GFP-LC puncta in confocal microscopy, and increased number of double-membrane autophagic vacuoles in electron micrographs. Hypoxic exposure also induced resistance of cancer cells to cisplatin, and LC3B siRNA restored the sensitivity of cancer cells to chemotherapy. Furthermore, Human lung cancer tissues that experienced chemotherapy showed increase of LC3BI to LC3BII conversion and decrease of p62/sequestosome1 compared with chemo-naïve cancer tissue in Western blot. CONCLUSION: Autophagy may play an important role in acquiring resistance to chemotherapy in lung cancer and hypoxia related pathway seems to be involved in autophagy induction.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherBioMed Central Ltd-
dc.relation.isPartOfRESPIRATORY RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdaptor Proteins, Signal Transducing/metabolism-
dc.subject.MESHAntineoplastic Agents/pharmacology*-
dc.subject.MESHAutophagy/drug effects*-
dc.subject.MESHCarcinoma, Non-Small-Cell Lung/drug therapy*-
dc.subject.MESHCarcinoma, Non-Small-Cell Lung/genetics-
dc.subject.MESHCarcinoma, Non-Small-Cell Lung/metabolism-
dc.subject.MESHCarcinoma, Non-Small-Cell Lung/ultrastructure-
dc.subject.MESHCell Hypoxia-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCisplatin/pharmacology*-
dc.subject.MESHDose-Response Relationship, Drug-
dc.subject.MESHDrug Resistance, Neoplasm*-
dc.subject.MESHHumans-
dc.subject.MESHLung Neoplasms/drug therapy*-
dc.subject.MESHLung Neoplasms/genetics-
dc.subject.MESHLung Neoplasms/metabolism-
dc.subject.MESHLung Neoplasms/ultrastructure-
dc.subject.MESHMicrotubule-Associated Proteins/genetics-
dc.subject.MESHMicrotubule-Associated Proteins/metabolism-
dc.subject.MESHRNA Interference-
dc.subject.MESHSequestosome-1 Protein-
dc.subject.MESHTime Factors-
dc.subject.MESHTransfection-
dc.subject.MESHTumor Microenvironment*-
dc.titleAutophagy contributes to the chemo-resistance of non-small cell lung cancer in hypoxic conditions-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Thoracic & Cardiovascular Surgery-
dc.contributor.googleauthorJin Gu Lee-
dc.contributor.googleauthorJu Hye Shin-
dc.contributor.googleauthorHyo Sup Shim-
dc.contributor.googleauthorChang Young Lee-
dc.contributor.googleauthorDae Joon Kim-
dc.contributor.googleauthorYoung Sam Kim-
dc.contributor.googleauthorKyung Young Chung-
dc.identifier.doi10.1186/s12931-015-0285-4-
dc.contributor.localIdA00368-
dc.contributor.localIdA00707-
dc.contributor.localIdA02155-
dc.contributor.localIdA02219-
dc.contributor.localIdA03225-
dc.contributor.localIdA03245-
dc.contributor.localIdA03571-
dc.relation.journalcodeJ02616-
dc.identifier.eissn1465-993X-
dc.identifier.pmid26553068-
dc.contributor.alternativeNameKim, Dae Joon-
dc.contributor.alternativeNameKim, Young Sam-
dc.contributor.alternativeNameShin, Ju Hye-
dc.contributor.alternativeNameShim, Hyo Sup-
dc.contributor.alternativeNameLee, Jin Gu-
dc.contributor.alternativeNameLee, Chang Young-
dc.contributor.alternativeNameChung, Kyung Young-
dc.contributor.affiliatedAuthorKim, Dae Joon-
dc.contributor.affiliatedAuthorKim, Young Sam-
dc.contributor.affiliatedAuthorShin, Ju Hye-
dc.contributor.affiliatedAuthorShim, Hyo Sup-
dc.contributor.affiliatedAuthorLee, Jin Gu-
dc.contributor.affiliatedAuthorLee, Chang Young-
dc.contributor.affiliatedAuthorChung, Kyung Young-
dc.citation.volume9-
dc.citation.number16-
dc.citation.startPage138-
dc.identifier.bibliographicCitationRESPIRATORY RESEARCH, Vol.9(16) : 138, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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