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Host Langerin (CD207) is a receptor for Yersinia pestis phagocytosis and promotes dissemination

Authors
 Kun Yang  ;  Chae G Park  ;  Cheolho Cheong  ;  Silvia Bulgheresi  ;  Shusheng Zhang  ;  Pei Zhang  ;  Yingxia He  ;  Lingyu Jiang  ;  Hongping Huang  ;  Honghui Ding  ;  Yiping Wu  ;  Shaogang Wang  ;  Lin Zhang  ;  Anyi Li  ;  Lianxu Xia  ;  Sara S Bartra  ;  Gregory V Plano  ;  Mikael Skurnik  ;  John D Klena  ;  Tie Chen 
Citation
 IMMUNOLOGY AND CELL BIOLOGY, Vol.93(9) : 815-824, 2015 
Journal Title
 IMMUNOLOGY AND CELL BIOLOGY 
ISSN
 0818-9641 
Issue Date
2015
MeSH
Animals ; Antigen-Presenting Cells/immunology* ; Antigen-Presenting Cells/microbiology ; Antigens, CD/immunology* ; Antigens, CD/metabolism ; Bacterial Adhesion/immunology ; CHO Cells ; Cells, Cultured ; Cricetinae ; Cricetulus ; Dendritic Cells/immunology ; Dendritic Cells/metabolism ; Flow Cytometry ; Host-Pathogen Interactions/immunology ; Humans ; Langerhans Cells/immunology ; Langerhans Cells/metabolism ; Lectins, C-Type/immunology* ; Lectins, C-Type/metabolism ; Mannose-Binding Lectins/immunology* ; Mannose-Binding Lectins/metabolism ; Mice ; O Antigens/immunology ; O Antigens/metabolism ; Phagocytosis/immunology* ; Plague/immunology ; Plague/microbiology ; Protein Binding/immunology ; Receptors, Cell Surface/immunology ; Receptors, Cell Surface/metabolism ; Survival Analysis ; Yersinia pestis/immunology* ; Yersinia pestis/metabolism ; Yersinia pestis/physiology
Abstract
Yersinia pestis is a Gram-negative bacterium that causes plague. After Y. pestis overcomes the skin barrier, it encounters antigen-presenting cells (APCs), such as Langerhans and dendritic cells. They transport the bacteria from the skin to the lymph nodes. However, the molecular mechanisms involved in bacterial transmission are unclear. Langerhans cells (LCs) express Langerin (CD207), a calcium-dependent (C-type) lectin. Furthermore, Y. pestis possesses exposed core oligosaccharides. In this study, we show that Y. pestis invades LCs and Langerin-expressing transfectants. However, when the bacterial core oligosaccharides are shielded or truncated, Y. pestis propensity to invade Langerhans and Langerin-expressing cells decreases. Moreover, the interaction of Y. pestis with Langerin-expressing transfectants is inhibited by purified Langerin, a DC-SIGN (DC-specific intercellular adhesion molecule 3 grabbing nonintegrin)-like molecule, an anti-CD207 antibody, purified core oligosaccharides and several oligosaccharides. Furthermore, covering core oligosaccharides reduces the mortality associated with murine infection by adversely affecting the transmission of Y. pestis to lymph nodes. These results demonstrate that direct interaction of core oligosaccharides with Langerin facilitates the invasion of LCs by Y. pestis. Therefore, Langerin-mediated binding of Y. pestis to APCs may promote its dissemination and infection.
Files in This Item:
T201504450.pdf Download
DOI
10.1038/icb.2015.46
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Park, Chae Gyu(박채규) ORCID logo https://orcid.org/0000-0003-1906-1308
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/156732
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