103 83

Cited 10 times in

Insufficient Discriminatory Power of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Dendrograms to Determine the Clonality of Multi-Drug-Resistant Acinetobacter baumannii Isolates from an Intensive Care Unit

 John Hoon Rim  ;  Yangsoon Lee  ;  Sung Kuk Hong  ;  Yongjung Park  ;  MyungSook Kim  ;  Roshan D'Souza  ;  Eun Suk Park  ;  Dongeun Yong  ;  Kyungwon Lee 
 BIOMED RESEARCH INTERNATIONAL, Vol.2015 : 535027, 2015 
Journal Title
Issue Date
Acinetobacter baumannii/genetics* ; Acinetobacter baumannii/isolation & purification* ; Acinetobacter baumannii/pathogenicity ; Drug Resistance, Multiple/genetics* ; Electrophoresis, Gel, Pulsed-Field ; Humans ; Intensive Care Units ; Species Specificity ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*
While pulsed-field gel electrophoresis (PFGE) is recognized as the gold standard method for clonality analysis, MALDI-TOF MS has recently been spotlighted as an alternative tool for species identification. Herein, we compared the dendrograms of multi-drug-resistant (MDR) Acinetobacter baumannii isolates by using MALDI-TOF MS with those by using PFGE. We used direct colony and protein extraction methods for MALDI-TOF MS dendrograms. The isolates with identical PFGE patterns were grouped into different branches in MALDI-TOF MS dendrograms. Among the isolates that were classified as very close isolates in MALDI-TOF MS dendrogram, PFGE band patterns visually showed complete differences. We numeralized similarity among isolates by measuring distance levels. The Spearman rank correlation coefficient values were 0.449 and 0.297 between MALDI-TOF MS dendrogram using direct colony and protein extraction method versus PFGE, respectively. This study is the first paper focusing solely on the dendrogram function of MALDI-TOF MS compared with PFGE. Although MALDI-TOF MS is a promising tool to identify species in a rapid manner, our results showed that MALDI-TOF MS dendrograms could not substitute PFGE for MDR Acinetobacter baumannii clonality analysis.
Files in This Item:
T201506511.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Rim, John Hoon(임정훈) ORCID logo https://orcid.org/0000-0001-6825-8479
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.