Cited 25 times in
Novel vaccine potential of Rv3131, a DosR regulon-encoded putative nitroreductase, against hyper-virulent Mycobacterium tuberculosis strain K
DC Field | Value | Language |
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dc.contributor.author | 남기택 | - |
dc.contributor.author | 신성재 | - |
dc.contributor.author | 조상래 | - |
dc.date.accessioned | 2017-11-02T08:35:28Z | - |
dc.date.available | 2017-11-02T08:35:28Z | - |
dc.date.issued | 2017 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/154650 | - |
dc.description.abstract | Accumulating evidence indicates that latency-associated Mycobacterium tuberculosis (Mtb)-specific antigens from the dormancy survival regulator regulon (DosR) may be promising novel vaccine target antigens for the development of an improved tuberculosis vaccine. After transcriptional profiling of DosR-related genes in the hyper-virulent Beijing Mtb strain K and the reference Mtb strain H37Rv, we selected Rv3131, a hypothetical nitroreductase, as a vaccine antigen and evaluated its vaccine efficacy against Mtb K. Mtb K exhibited stable and constitutive up-regulation of rv3131 relative to Mtb H37Rv under three different growth conditions (at least 2-fold induction) including exponential growth in normal culture conditions, hypoxia, and inside macrophages. Mice immunised with Rv3131 formulated in GLA-SE, a well-defined TLR4 adjuvant, displayed enhanced Rv3131-specific IFN-γ and serum IgG2c responses along with effector/memory T cell expansion and remarkable generation of Rv3131-specific multifunctional CD4+ T cells co-producing TNF-α, IFN-γ and IL-2 in both spleen and lung. Following challenge with Mtb K, the Rv3131/GLA-SE-immunised group exhibited a significant reduction in bacterial number and less extensive lung inflammation accompanied by the obvious persistence of Rv3131-specific multifunctional CD4+ T cells. These results suggest that Rv3131 could be an excellent candidate for potential use in a multi-antigenic Mtb subunit vaccine, especially against Mtb Beijing strains. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Novel vaccine potential of Rv3131, a DosR regulon-encoded putative nitroreductase, against hyper-virulent Mycobacterium tuberculosis strain K | - |
dc.type | Article | - |
dc.publisher.location | England | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Life Science | - |
dc.contributor.googleauthor | Kee Woong Kwon | - |
dc.contributor.googleauthor | Woo Sik Kim | - |
dc.contributor.googleauthor | Hongmin Kim | - |
dc.contributor.googleauthor | Seung Jung Han | - |
dc.contributor.googleauthor | Mi-Young Hahn | - |
dc.contributor.googleauthor | Jong Seok Lee | - |
dc.contributor.googleauthor | Ki Taek Nam | - |
dc.contributor.googleauthor | Sang-Nae Cho | - |
dc.contributor.googleauthor | Sung Jae Shin | - |
dc.identifier.doi | 10.1038/srep44151 | - |
dc.contributor.localId | A02114 | - |
dc.contributor.localId | A03824 | - |
dc.contributor.localId | A01243 | - |
dc.relation.journalcode | J02646 | - |
dc.identifier.eissn | 2045-2322 | - |
dc.identifier.pmid | 28272457 | - |
dc.contributor.alternativeName | Nam, Ki Taek | - |
dc.contributor.alternativeName | Shin, Sung Jae | - |
dc.contributor.alternativeName | Cho, Sang Nae | - |
dc.contributor.affiliatedAuthor | Shin, Sung Jae | - |
dc.contributor.affiliatedAuthor | Cho, Sang Nae | - |
dc.contributor.affiliatedAuthor | Nam, Ki Taek | - |
dc.citation.title | Scientific Reports | - |
dc.citation.volume | 7 | - |
dc.citation.startPage | 44151 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, Vol.7 : 44151, 2017 | - |
dc.date.modified | 2017-11-01 | - |
dc.identifier.rimsid | 43707 | - |
dc.type.rims | ART | - |
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