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The DNA aptamer binds stemness-enriched cancer cells in pancreatic cancer

DC FieldValueLanguage
dc.contributor.author송시영-
dc.contributor.author이희승-
dc.contributor.author김정미-
dc.date.accessioned2017-11-02T08:30:51Z-
dc.date.available2017-11-02T08:30:51Z-
dc.date.issued2017-
dc.identifier.issn0952-3499-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/154553-
dc.description.abstractPancreatic cancer remains one of the most common and lethal cancers. Most patients (80%) present with inoperable advanced pancreatic cancer at initial diagnosis, and their early diagnosis is a significant unmet challenge. Recent studies indicate that cancer, including pancreatic cancer, is initiated and propagated by cancer stem cells (CSCs). CSCs are responsible not only for the pathogenesis of cancer but also for the heterogeneity, malignant degree, anticancer therapy resistance, and recurrence of tumors. Therefore, the identification of CSCs may be a crucial stepping stone for overcoming this disastrous pancreatic cancer. Here, we investigated pancreatic CSC-associated aptamers as a novel tool for diagnosis and therapeutic agents. Aptamers that bind to stemness-enriched cancer cells in pancreatic cancer were developed by modified Cell-SELEX method. Positive selection was performed by the sphere cells generated by pancreatic cancer cell line, HPAC, and then the aptamer pool was negatively selected by pancreatic normal cell line, HPDE. Aptamers 1 and 146 showing high specificity upon the KD values with 22.18 and 22.62 nM were selected. These 2 aptamers were validated by binding to HPAC sphere cells and to HPDE cells, and both aptamers showed specificity to HPAC sphere cells only. Aptamer-positive cells showed high expression levels of CSC-associated genes compared with the aptamer-negative cells by FACS analysis. The colocalization of CD44, CD24, ESA, and CD133 was also observed in the aptamer-positive cells by confocal microscopy. In the present study, these 2 pancreatic CSC-associated aptamers may be potential candidates for novel diagnostic markers, CSC-targeting drug delivery, or circulating tumor cell detection.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherJohn Wiley & Sons-
dc.relation.isPartOfJOURNAL OF MOLECULAR RECOGNITION-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAC133 Antigen/metabolism-
dc.subject.MESHAptamers, Nucleotide/chemistry-
dc.subject.MESHAptamers, Nucleotide/metabolism*-
dc.subject.MESHBiomarkers, Tumor/metabolism-
dc.subject.MESHCD24 Antigen/metabolism-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHHumans-
dc.subject.MESHHyaluronan Receptors/metabolism-
dc.subject.MESHNeoplastic Stem Cells/metabolism*-
dc.subject.MESHPancreatic Neoplasms/metabolism*-
dc.subject.MESHSELEX Aptamer Technique-
dc.titleThe DNA aptamer binds stemness-enriched cancer cells in pancreatic cancer-
dc.typeArticle-
dc.publisher.locationEngland-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorYoon-Jin Kim-
dc.contributor.googleauthorHee Seung Lee-
dc.contributor.googleauthorDawoon E. Jung-
dc.contributor.googleauthorJeong Mi Kim-
dc.contributor.googleauthorSi Young Song-
dc.identifier.doi10.1002/jmr.2591-
dc.contributor.localIdA03349-
dc.contributor.localIdA00882-
dc.contributor.localIdA02035-
dc.relation.journalcodeJ03079-
dc.identifier.eissn1099-1352-
dc.relation.journalsince1988-
dc.identifier.pmid27891685-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/jmr.2591/abstract-
dc.subject.keywordaptamer-
dc.subject.keywordcancer stem cell-
dc.subject.keywordcirculating tumor cell-
dc.subject.keywordpancreatic cancer-
dc.contributor.alternativeNameSong, Si Young-
dc.contributor.alternativeNameLee, Hee Seung-
dc.contributor.alternativeNameKim, Jeong Mi-
dc.contributor.affiliatedAuthorLee, Hee Seung-
dc.contributor.affiliatedAuthorKim, Jeong Mi-
dc.contributor.affiliatedAuthorSong, Si Young-
dc.citation.titleJournal of Molecular Recognition-
dc.citation.volume30-
dc.citation.number4-
dc.citation.startPage2591-
dc.identifier.bibliographicCitationJOURNAL OF MOLECULAR RECOGNITION, Vol.30(4) : 2591, 2017-
dc.date.modified2017-11-01-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Gastroenterology (소화기병연구소) > 1. Journal Papers

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