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Effect of botulinum toxin A on vasoconstriction and sympathetic neurotransmitters in a murine random pattern skin flap model

DC Field Value Language
dc.contributor.author김영석-
dc.contributor.author노태석-
dc.contributor.author유대현-
dc.contributor.author윤인식-
dc.contributor.author정복기-
dc.date.accessioned2017-11-02T08:26:37Z-
dc.date.available2017-11-02T08:26:37Z-
dc.date.issued2017-
dc.identifier.issn1067-1927-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/154474-
dc.description.abstractBlood supply is the most important factor determining the survival of a skin flap. Botulinum toxin-A (Botox-A) is used as pharmacologic agent not only for aesthetic purposes, but also for its vasomotor actions. This study was conducted to establish whether local application of Botox-A increased survival of random pattern skin flaps in rats by changing the expression of neurotransmitters. Forty adult Sprague-Dawley rats with a caudally-based random pattern skin flap were divided into two groups: Botox-A group and saline group. Surviving flap area and cutaneous blood flow in the flap were evaluated on postoperative days 3 and 7. After injection of Botox-A, changes in vessels were analyzed using immunohistochemical staining. Levels of norepinephrine, neuropeptide-Y, nitric oxide, and endothelial nitric oxide synthase were analyzed quantitatively by high performance liquid chromatography, Western blot, and colorimetric assay. The survived area in the Botox-A group was significantly higher than that in the control group on postoperative days 3 and 7. Blood flow in the Botox-A group was significantly high in the proximal and middle areas immediately after the operation. The number of CD31-positive vessels in the Botox-A group was significant greater than that in the control group. Norepinephrine level in the Botox-A group decreased significantly immediately after flap elevation and at postoperative day 3. There were no significant differences in neuropeptide-Y level between the two groups. Nitric oxide level did not change significantly in either group despite the increase in endothelial nitric oxide synthase immediately after flap elevation and at 3 days postoperatively. In conclusion, Botox-A increased vascular blood flow and viable flap area in rats by reducing norepinephrine level. In contrast, neuropeptide-Y, another vasoconstrictor, was not affected by Botox-A. Nitric oxide, a vasodilator, was also not affected by Botox-A, despite the significant increase in endothelial nitric oxide synthase expression in the flaps.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherBlackwell Science-
dc.relation.isPartOfWOUND REPAIR AND REGENERATION-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBotulinum Toxins, Type A/administration & dosage*-
dc.subject.MESHCardiovascular Agents/administration & dosage*-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHImmunohistochemistry-
dc.subject.MESHInjections-
dc.subject.MESHNeuropeptide Y/analysis-
dc.subject.MESHNeuropeptide Y/metabolism-
dc.subject.MESHNeurotransmitter Agents/biosynthesis*-
dc.subject.MESHNitric Oxide/analysis-
dc.subject.MESHNitric Oxide/metabolism-
dc.subject.MESHNitric Oxide Synthase Type III/analysis-
dc.subject.MESHNitric Oxide Synthase Type III/metabolism-
dc.subject.MESHNorepinephrine/analysis-
dc.subject.MESHNorepinephrine/metabolism-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHSkin/blood supply-
dc.subject.MESHSkin/drug effects*-
dc.subject.MESHSkin/physiopathology-
dc.subject.MESHSurgical Flaps/blood supply*-
dc.subject.MESHSympathomimetics/metabolism-
dc.subject.MESHVasoconstriction/drug effects-
dc.subject.MESHVasoconstriction/physiology-
dc.subject.MESHWound Healing/drug effects*-
dc.subject.MESHWound Healing/physiology-
dc.titleEffect of botulinum toxin A on vasoconstriction and sympathetic neurotransmitters in a murine random pattern skin flap model-
dc.typeArticle-
dc.publisher.locationUnited States-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Plastic Surgery & Reconstructive Surgery-
dc.contributor.googleauthorTai Suk Roh-
dc.contributor.googleauthorBok Ki Jung-
dc.contributor.googleauthorInsik Yun-
dc.contributor.googleauthorDae Hyun Lew-
dc.contributor.googleauthorYoung Seok Kim-
dc.identifier.doi10.1111/wrr.12501-
dc.contributor.localIdA01297-
dc.contributor.localIdA02459-
dc.contributor.localIdA02588-
dc.contributor.localIdA04665-
dc.contributor.localIdA00709-
dc.relation.journalcodeJ02807-
dc.identifier.eissn1524-475X-
dc.identifier.pmid27997734-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/wrr.12501/abstract-
dc.contributor.alternativeNameKim, Young Seok-
dc.contributor.alternativeNameRoh, Tai Suk-
dc.contributor.alternativeNameLew, Dae Hyun-
dc.contributor.alternativeNameYun, In Sik-
dc.contributor.alternativeNameJung, Bok Ki-
dc.contributor.affiliatedAuthorRoh, Tai Suk-
dc.contributor.affiliatedAuthorLew, Dae Hyun-
dc.contributor.affiliatedAuthorYun, In Sik-
dc.contributor.affiliatedAuthorJung, Bok Ki-
dc.contributor.affiliatedAuthorKim, Young Seok-
dc.citation.titleWound Repair and Regeneration-
dc.citation.volume25-
dc.citation.number1-
dc.citation.startPage75-
dc.citation.endPage85-
dc.identifier.bibliographicCitationWOUND REPAIR AND REGENERATION, Vol.25(1) : 75-85, 2017-
dc.date.modified2017-11-01-
dc.identifier.rimsid43048-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers

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