Cited 23 times in
Effect of botulinum toxin A on vasoconstriction and sympathetic neurotransmitters in a murine random pattern skin flap model
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김영석 | - |
dc.contributor.author | 노태석 | - |
dc.contributor.author | 유대현 | - |
dc.contributor.author | 윤인식 | - |
dc.contributor.author | 정복기 | - |
dc.date.accessioned | 2017-11-02T08:26:37Z | - |
dc.date.available | 2017-11-02T08:26:37Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 1067-1927 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/154474 | - |
dc.description.abstract | Blood supply is the most important factor determining the survival of a skin flap. Botulinum toxin-A (Botox-A) is used as pharmacologic agent not only for aesthetic purposes, but also for its vasomotor actions. This study was conducted to establish whether local application of Botox-A increased survival of random pattern skin flaps in rats by changing the expression of neurotransmitters. Forty adult Sprague-Dawley rats with a caudally-based random pattern skin flap were divided into two groups: Botox-A group and saline group. Surviving flap area and cutaneous blood flow in the flap were evaluated on postoperative days 3 and 7. After injection of Botox-A, changes in vessels were analyzed using immunohistochemical staining. Levels of norepinephrine, neuropeptide-Y, nitric oxide, and endothelial nitric oxide synthase were analyzed quantitatively by high performance liquid chromatography, Western blot, and colorimetric assay. The survived area in the Botox-A group was significantly higher than that in the control group on postoperative days 3 and 7. Blood flow in the Botox-A group was significantly high in the proximal and middle areas immediately after the operation. The number of CD31-positive vessels in the Botox-A group was significant greater than that in the control group. Norepinephrine level in the Botox-A group decreased significantly immediately after flap elevation and at postoperative day 3. There were no significant differences in neuropeptide-Y level between the two groups. Nitric oxide level did not change significantly in either group despite the increase in endothelial nitric oxide synthase immediately after flap elevation and at 3 days postoperatively. In conclusion, Botox-A increased vascular blood flow and viable flap area in rats by reducing norepinephrine level. In contrast, neuropeptide-Y, another vasoconstrictor, was not affected by Botox-A. Nitric oxide, a vasodilator, was also not affected by Botox-A, despite the significant increase in endothelial nitric oxide synthase expression in the flaps. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Blackwell Science | - |
dc.relation.isPartOf | WOUND REPAIR AND REGENERATION | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Botulinum Toxins, Type A/administration & dosage* | - |
dc.subject.MESH | Cardiovascular Agents/administration & dosage* | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Injections | - |
dc.subject.MESH | Neuropeptide Y/analysis | - |
dc.subject.MESH | Neuropeptide Y/metabolism | - |
dc.subject.MESH | Neurotransmitter Agents/biosynthesis* | - |
dc.subject.MESH | Nitric Oxide/analysis | - |
dc.subject.MESH | Nitric Oxide/metabolism | - |
dc.subject.MESH | Nitric Oxide Synthase Type III/analysis | - |
dc.subject.MESH | Nitric Oxide Synthase Type III/metabolism | - |
dc.subject.MESH | Norepinephrine/analysis | - |
dc.subject.MESH | Norepinephrine/metabolism | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Skin/blood supply | - |
dc.subject.MESH | Skin/drug effects* | - |
dc.subject.MESH | Skin/physiopathology | - |
dc.subject.MESH | Surgical Flaps/blood supply* | - |
dc.subject.MESH | Sympathomimetics/metabolism | - |
dc.subject.MESH | Vasoconstriction/drug effects | - |
dc.subject.MESH | Vasoconstriction/physiology | - |
dc.subject.MESH | Wound Healing/drug effects* | - |
dc.subject.MESH | Wound Healing/physiology | - |
dc.title | Effect of botulinum toxin A on vasoconstriction and sympathetic neurotransmitters in a murine random pattern skin flap model | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Plastic Surgery & Reconstructive Surgery | - |
dc.contributor.googleauthor | Tai Suk Roh | - |
dc.contributor.googleauthor | Bok Ki Jung | - |
dc.contributor.googleauthor | Insik Yun | - |
dc.contributor.googleauthor | Dae Hyun Lew | - |
dc.contributor.googleauthor | Young Seok Kim | - |
dc.identifier.doi | 10.1111/wrr.12501 | - |
dc.contributor.localId | A01297 | - |
dc.contributor.localId | A02459 | - |
dc.contributor.localId | A02588 | - |
dc.contributor.localId | A04665 | - |
dc.contributor.localId | A00709 | - |
dc.relation.journalcode | J02807 | - |
dc.identifier.eissn | 1524-475X | - |
dc.identifier.pmid | 27997734 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/wrr.12501/abstract | - |
dc.contributor.alternativeName | Kim, Young Seok | - |
dc.contributor.alternativeName | Roh, Tai Suk | - |
dc.contributor.alternativeName | Lew, Dae Hyun | - |
dc.contributor.alternativeName | Yun, In Sik | - |
dc.contributor.alternativeName | Jung, Bok Ki | - |
dc.contributor.affiliatedAuthor | Roh, Tai Suk | - |
dc.contributor.affiliatedAuthor | Lew, Dae Hyun | - |
dc.contributor.affiliatedAuthor | Yun, In Sik | - |
dc.contributor.affiliatedAuthor | Jung, Bok Ki | - |
dc.contributor.affiliatedAuthor | Kim, Young Seok | - |
dc.citation.title | Wound Repair and Regeneration | - |
dc.citation.volume | 25 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 75 | - |
dc.citation.endPage | 85 | - |
dc.identifier.bibliographicCitation | WOUND REPAIR AND REGENERATION, Vol.25(1) : 75-85, 2017 | - |
dc.date.modified | 2017-11-01 | - |
dc.identifier.rimsid | 43048 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.