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Control of dental-derived induced pluripotent stem cells through modified surfaces for dental application

Authors
 Hyunmin Choi  ;  Kyu-Hyung Park  ;  Ah-Reum Lee  ;  Chin Hee Mun  ;  Yong Dae Shin  ;  Yong-Beom Park  ;  Young-Bum Park 
Citation
 ACTA ODONTOLOGICA SCANDINAVICA, Vol.75(5) : 309-318, 2017 
Journal Title
ACTA ODONTOLOGICA SCANDINAVICA
ISSN
 0001-6357 
Issue Date
2017
MeSH
Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Dental Implants ; Fibroblasts/physiology* ; Gingiva/cytology ; Humans ; Induced Pluripotent Stem Cells/drug effects ; Induced Pluripotent Stem Cells/metabolism* ; Osteogenesis ; Surface Properties ; Titanium/pharmacology
Keywords
Induced pluripotent stem cells derived from dental stem cells ; characterization ; osteogenesis ; rough surface ; titanium disc
Abstract
OBJECTIVE: The aim of this study is to investigate the behaviour of iPSc derived from dental stem cells in terms of initial adhesion, differentiation potential on differently surface-treated titanium disc.

MATERIALS AND METHODS: iPSc derived from human gingival fibroblasts (hGFs) were established using 4-reprogramming factors transduction with Sendai virus. The hGF-iPSc established in this study exhibited the morphology and growth properties similar to human embryonic stem (ES) cells and expressed pluripotency makers. Alkaline Phosphatase (AP) staining, Embryoid Body (EB) formation and in vitro differentiation and karyotyping further confirmed pluripotency of hGF-iPSc. Then, hGF-iPSc were cultured on machined- and Sandblasted and acid etched (SLA)-treated titanium discs with osteogenic induction medium and their morphological as well as quantitative changes according to different surface types were investigated using Alizrin Red S staining, Scanning electron microscopy (SEM), Flow cytometry and RT-PCR.

RESULTS: Time-dependent and surface-dependent morphological changes as well as quantitative change in osteogenic differentiation of hGF-iPSc were identified and osteogenic gene expression of hGF-iPSc cultured on SLA-treated titanium disc found to be greater than machined titanium disc, suggesting the fate of hGF-iPSc may be determined by the characteristics of surface to which hGF-iPSc first adhere.

CONCLUSIONS: iPSc derived from dental stem cell can be one of the most promising and practical cell sources for personalized regenerative dentistry and their morphological change as well as quantitative change in osteogenic differentiation according to different surface types may be further utilized for future clinical application incorporated with dental implant.
Full Text
http://www.tandfonline.com/doi/full/10.1080/00016357.2017.1303847
DOI
10.1080/00016357.2017.1303847
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Prosthodontics (보철과학교실) > 1. Journal Papers
Yonsei Authors
Park, Young Bum(박영범)
Park, Yong Beom(박용범)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154420
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